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Translation

Protein structures. tutorial. Translation. Stryer Ch 29 Alberts pp. 331-373 and Ch 12. MVA Fig. 27.19 Assembly of 30S subunit. ATP. Activation! E.coli tRNA gln. rRNA synthesis is affected by nutrition:. Low aa availability is signalled by a ribosome bound to an uncharged tRNA.

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Translation

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  1. Protein structures tutorial Translation Stryer Ch 29 Alberts pp. 331-373 and Ch 12

  2. MVA Fig. 27.19 Assembly of 30S subunit.

  3. ATP Activation! E.coli tRNAgln

  4. rRNA synthesis is affected by nutrition: Low aa availability is signalled by a ribosome bound to an uncharged tRNA. This stimulates a protein called “stringent factor” to catalyze synthesis of the unusual nucleotide ppGpp. This intracellular messenger reduces transcription of rRNA and tRNA genes while upregulating genes involved in aa biosynthesis while repressing genes for replication and carbohydrate synthesis. How does it work? It acts on RNA pol to affect promoter specifity.

  5. (frame shift corrected)

  6. Initiation

  7. Initiation of Translation

  8. Interactions based on x-linking

  9. Interactions based on x-linking

  10. From Science 285: 2095, 1999. CCA end ^ *

  11. Clamp in 30S subunit holding P-site tRNA, and50 S tRNA in P site Codon-anticodon binding A-site tRNA

  12. Opposite views mRNA Grey= 50S, Switch helix= light blue, 30S = purple, A-site tRNA=red

  13. Cognate recoginition

  14. Ribosomal binding sites in the elongation cycle.

  15. Termination

  16. Energetics: 4 high energy bonds/aa YOU do the math!!!

  17. Termination

  18. Kinetic Proofreading for Selecting Codon-Anticodon Binding

  19. Secretory Pathway

  20. Human globin genes. Red = active, green = pseudogenes, yellow = Kpn sequences

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