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Transcriptional regulatory code of a eukaryotic genome

Transcriptional regulatory code of a eukaryotic genome. Harbison CT et al. Nature , 2004, Vol. 431 , pp. 99-104 Milica Volar 3 March 2005. Outline of talk. Background Yeast’s transcriptional regulatory map Promoter structure Regulator binding behaviour Summary.

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Transcriptional regulatory code of a eukaryotic genome

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  1. Transcriptional regulatory code of a eukaryotic genome Harbison CT et al. Nature, 2004, Vol. 431, pp. 99-104 Milica Volar 3 March 2005.

  2. Outline of talk • Background • Yeast’s transcriptional regulatory map • Promoter structure • Regulator binding behaviour • Summary

  3. Transcriptional regulators • Proteins that bind to specific promoter sequences in genome • Regulate genome expression and its maintenance • Cell cycle, environment changes, replication, condensation, cohesion, etc.

  4. Procedure

  5. Genome wide location analysis Crosslink protein to DNA in vivo with formaldehyde Break open cells and shear DNA Immunoprecipitate Reverse-crosslinks, blunt DNA and ligate to unidirectional linkers LM-PCR Labeling Hybridize to array Ren B et al, 2004

  6. Procedure They tested the binding by GWLA: 203 (84) DNA transcriptional regulators in rich media -> 11,000 unique interactions between regulators and promoter regions at high confidence (P<0.001)

  7. Yeast transcriptional regulatory map

  8. Yeast transcriptional regulatory map 3,353 interactions within 1,296 promoter regions

  9. Promoter architectures

  10. Promoter architectures

  11. Regulator binding behaviour

  12. Regulator binding behaviour

  13. Summary • Construction of the yeast transcriptional regulatory map • Promoter architectures • Patterns of regulator binding behaviour

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