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What is a Transcriptional Regulatory Network (TRN)?

Characterization of Transcriptional Regulatory Networks controlling plant cell adaptation to environmental stresses. What is a Transcriptional Regulatory Network (TRN)?. It’s a map of gene expression programs. These programs control plant development and plant adaptation to stresses.

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What is a Transcriptional Regulatory Network (TRN)?

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  1. Characterization of Transcriptional Regulatory Networks controlling plant cell adaptation to environmental stresses

  2. What is aTranscriptional Regulatory Network (TRN)? It’s a map of gene expression programs. These programs control plant development and plant adaptation to stresses. These programs depend on the recognition of specific promoter sequences by transcriptional regulatory proteins as well as epigenetic modifications of the genetic material.

  3. The characterization of plant TRNs requires the qualitative and quantitative integration of inter-disciplinary biological approaches Nuclear Protein-DNA interactions (selected TFs) Nuclear protein-protein interactions (selected TFs) Small RNAs Genomic DNA methylome (cytosine methylation pattern) Transcriptome (RNA-sequencing) Hisotne modifications Bioinformatics Transcriptional Regulatory Networks

  4. The cellular complexity of plant roots is not suitable to characterize TRNs. Rt=6t1+3t2+6t3+4t4+1t5 t’1 t1 t’3 t3 t’5 t5 t’4 t4 t’2 t2 Environmental stress Rt’=6t’1+3t’2+6t’3+4t’4+1t’5 A change between Rt and Rt’ might reflect: 1- a global change across all the different cell types of the analyzed tissue/organ t1≠t’1; t2 ≠ t’2; t3 ≠ t’3; t4 ≠ t’4; t5 ≠ t’5 2-a change of the transcriptome of a singe cell type. t1=t’1; t2 ≠ t’2; t3 = t’3; t4 = t’4; t5 = t’5

  5. A Single cell model is required to clearly characterize TRNs. Root hair cells are epidermal root cells characterized by their polar growth David Ponder (SEM picture)

  6. Use of an aeroponic system to treat roots and investigate the biology and TRNs of the root hair cell

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