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control siRNA. P115 siRNA. 0. 0.5. 1. 2. 0. 0.5. 1. 2. (min). thrombin. P115. Rho-GTP. Total Rho. A. B. GFP. PRG. P115. LARG. GFP. P115. +. +. +. +. +. +. +. Rac. Rac. +. +. +. +. +. +. +. +. +. +. PAK. PAK. IP-AU1 P115 WB PAK. IP-myc WB GFP. IP-myc
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control siRNA P115 siRNA 0 0.5 1 2 0 0.5 1 2 (min) thrombin P115 Rho-GTP Total Rho A B GFP PRG P115 LARG GFP P115 + + + + + + + Rac Rac + + + + + + + + + + PAK PAK IP-AU1 P115 WB PAK IP-myc WB GFP IP-myc WB PAK WB P115 TCL WB GFP WB PAK C D time 0 15 30 (min) Input PAK1 (32P) PAK AU1 IP (32P) FL p115 AU1 IP Coomassie FL p115