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AtISA1-FL

AtISA1-FL.

nyla
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AtISA1-FL

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  1. AtISA1-FL ATGGATGCAATCAAATGCAGTTCCAGTTTCCTCCATCACACCAAGCTCAACACTCTCTTCTCTAATCACACCTTTCCTAAAATCTCCGCTCCCAATTTCAAGCCCCTCTTCCGTCCGATTTCGATTTCCGCAAAGGACAGAAGAAGCAACGAAGCTGAAAATATCGCCGTAGTAGAGAAACCTCTTAAATCAGATAGATTTTTTATCTCCGATGGACTTCCTTCTCCATTCGGACCCACCGTCAGAGACGACGGCGTCAATTTCTCTGTTTACTCTACAAACTCCGTTTCCGCTACCATCTGCTTGATTTCTCTCTCCGATCTCCGTCAGAACAAAGTGACGGAGGAGATTCAGCTTGATCCATCAAGGAATAGAACTGGCCATGTTTGGCATGTGTTCTTGAGAGGAGATTTCAAAGATATGTTGTATGGTTATAGATTTGATGGCAAGTTTTCTCCTGAAGAAGGTCATTATTATGATTCCTCCAACATTTTATTGGATCCTTACGCAAAGGCAATTATAAGCAGAGATGAGTTTGGAGTTTTGGGACCTGATGATAATTGTTGGCCTCAAATGGCGTGTATGGTACCCACTCGTGAGGAAGAGTTTGATTGGGAAGGGGATATGCATCTGAAGCTTCCACAGAAAGATCTTGTTATATATGAAATGCATGTGCGAGGTTTTACAAGGCATGAGTCTAGTAAAATTGAATTCCCTGGCACATACCAGGGTGTTGCAGAGAAGCTTGACCATTTGAAGGAGCTTGGGATAAATTGTATAGAATTAATGCCATGTCACGAGTTTAATGAGCTGGAGTATTACAGCTACAATACGATTTTGGGAGACCACAGGGTAAATTTTTGGGGTTACTCTACCATTGGGTTCTTCTCGCCCATGATCAGATACGCATCAGCAAGCTCTAACAATTTTGCTGGACGAGCCATAAATGAATTCAAAATTCTTGTTAAGGAGGCACATAAACGAGGAATTGAGGTAATCATGGATGTCGTCTTGAACCACACAGCCGAAGGGAATGAAAAAGGGCCCATTTTCTCATTTAGAGGAGTTGATAACAGTGTCTATTACATGCTTGCTCCAAAGGGCGAGTTCTATAATTATTCAGGCTGTGGTAATACATTCAACTGCAATCATCCTGTGGTGCGTCAATTCATATTGGATTGCCTGAGATATTGGGTTACAGAAATGCATGTTGACGGCTTCCGCTTTGATCTTGGTTCAATCATGTCAAGGAGCAGCAGCCTTTGGGATGCAGCCAATGTTTACGGGGCTGATGTAGAAGGTGACTTGCTCACAACTGGTACTCCTATTAGCTGCCCTCCAGTAATTGACATGATAAGTAATGATCCAATACTCCGCGGTGTTAAGCTAATAGCTGAAGCATGGGATGCGGGTGGGCTGTACCAAGTTGGCATGTTTCCACACTGGGGTATTTGGTCTGAGTGGAATGGAAAGTTTCGGGATGTTGTGAGACAGTTCATAAAAGGCACCGATGGCTTTTCTGGTGCTTTTGCTGAATGTCTCTGTGGAAGCCCAAATCTGTACCAGGGAGGTAGGAAACCTTGGCACAGCATCAATTTTATATGTGCGCATGATGGTTTTACGTTGGCAGATTTAGTAACTTACAACAATAAGAATAACTTGGCAAATGGAGAAGAGAATAATGATGGAGAGAATCACAATTACAGCTGGAACTGTGGAGAGGAGGGAGACTTTGCGAGTATCTCGGTGAAGAGACTAAGGAAACGACAGATGCGGAATTTCTTTGTTTCCCTCATGGTTTCCCAAGGTGTCCCAATGATTTACATGGGAGATGAATATGGCCATACTAAAGGGGGAAACAACAACACGTATTGCCATGACAACTATATGAACTATTTTCGGTGGGATAAAAAGGAAGAAGCACATTCTGACTTCTTCAGATTCTGCCGTATTCTTATCAAGTTTCGTGATGAATGTGAATCACTTGGCTTGAATGATTTCCCAACAGCAAAGCGTCTGCAGTGGCATGGTCTTGCTCCTGAGATCCCAAATTGGTCTGAAACAAGTCGATTTGTTGCATTTTCACTGGTCGATTCTGTGAAGAAAGAAATCTATGTGGCCTTCAACACCAGTCATTTAGCCACACTTGTTTCGCTACCGAATAGGCCAGGATACCGATGGGAGCCATTTGTAGACACGAGCAAACCGAGCCCTTACGACTGCATAACACCGGATCTCCCAGAGAGAGAAACGGCGATGAAGCAGTATAGGCACTTCTTAGATGCAAATGTGTACCCAATGCTCAGTTACTCATCCATCATTCTCCTTCTTTCACCAATTAAAGACCCCTGA ZmISA1-FL ATGGATGCAATCAAATGCAGTTCCAGTTTCCTCCATCACACCAAGCTCAACACTCTCTTCTCTAATCACACCTTTCCTAAAATCTCCGCTCCCAATTTCAAGCCCCTCTTCCGTCCGATTTCGATTTCCGTGGCCGAGGCGGTGCAGGCGGAGGAGGACGACGACGACGACGACGAGGAGGTGGCCGAGGAGAGGTTCGCGCTGGGCGGCGCGTGCCGGGTGCTCGCGGGAATGCCCGCGCCGCTCGGCGCCACCGCGCTCCGCGGCGGTGTCAACTTCGCCGTCTACTCCAGCGGTGCCTCCGCCGCGTCGCTGTGCCTCTTCGCTCCCGGCGACCTCAAGGCGGATAGGGTGACCGAGGAGGTGCCCCTCGATCCCCTGCTCAACCGAACGGGAAACGTGTGGCACGTGTTCATCCACGGGGACCAGCTGCACGGCATGCTCTACGGATACAGGTTCGATGGCGTGTTCGCCCCTGAGCGCGGACAGTACTACGATGTGTCCAACGTTGTGGTGGATCCATACGCTAAGGCAGTGGTAAGCCGAGGTGAATATGGTGTGCCTGCGCCTGGTGGTAGTTGTTGGCCTCAAATGGCTGGTATGATCCCTCTTCCCTATAATAAGTTTGATTGGCAAGGTGACCTACCCCTTGGGTACCATCAGAAGGACCTTGTCATATATGAAATGCATTTGCGTGGATTCACAAAGCACAACTCAAGCAAGACAAAACACCCAGGAACTTACATTGGTGCTGTGTCAAAGCTTGACCATCTAAAGGAACTTGGAGTGAACTGTATAGAGCTAATGCCCTGCCATGAGTTCAATGAGCTAGAGTACTTCAGCTCCTCTTCGAAGATGAACTTCTGGGGATATTCCACAATAAATTTTTTCTCACCAATGGCAAGATATTCTTCAAGTGGCATAAGAGACTCTGGATGTGGTGCCATAAATGAATTTAAAGCTTTTGTAAGGGAGGCCCACAAACGGGGAATTGAGGTGATCATGGATGTTGTCTTCAATCATACAGCTGAAGGTAATGAGAAAGGCCCAATATTATCCTTTAGGGGGATAGATAATAGTACATACTACATGCTTGCACCTAAGGGAGAGTTTTATAATTATTCTGGTTGTGGAAATACCTTCAATTGTAATCATCCTGTAGTCCGTGAATTTATAGTGGATTGCTTGAGATACTGGGTAACAGAAATGCATGTTGATGGTTTTCGTTTTGACCTTGCATCTATACTGACCAGAGGATGCAGTCTATGGGATCCAGTTAATGTGTATGGAAGTCCAATGGAAGGTGACATGATTACGACAGGGACACCTCTTGTTGCCCCACCACTTATTGACATGATTAGCAATGACCCAATTCTTGGAAATGTCAAGCTCATTGCTGAAGCATGGGATGCAGGAGGTCTCTATCAAGTTGGTCAGTTTCCTCACTGGAACGTTTGGTCAGAGTGGAATGGAAAGTATCGCGATACCGTGCGTCAGTTCATCAAAGGCACAGATGGATTTGCTGGTGCTTTTGCTGAATGCCTATGTGGAAGTCCACAGTTATACCAGGCAGGGGGGAGGAAGCCTTGGCACAGTATCAACTTTGTATGTGCACACGATGGATTTACACTGGCTGATTTGGTCACATACAATAGCAAGTACAACTTGTCAAATGGTGAGGACAACAGAGATGGGGAAAATCATAATCTTAGCTGGAATTGTGGGGAGGAAGGAGAATTTGCAAGTCTGTCAGTCCGAAGATTAAGGAAGAGGCAAATGCGCAATTTCTTTGTTTGTCTTATGGTTTCTCAGGGAGTTCCAATGTTCTACATGGGCGATGAATATGGTCACACAAAGGGAGGGAACAACAATACGTACTGCCATGACCATTATGTCAATTATTTCCGTTGGGATAAGAAGGAAGAACAATCCTCTGATTTGTACAGATTCTGCCGTCTCATGACCAAATTCCGCAAAGAATGTGAATCTCTTGGCCTTGAGGACTTCCCGACTTCAGAACGGTTGAAATGGCACGGTCATCAGCCCGAGAAGCCTGACTGGTCAGAGGCAAGCCGATTCGTTGCCTTCACCATGAAGGACGAAACCAAAGGCGAGATCTACGTGGCCTTCAACACCAGTCACCTTCCGGTGGTTGTTGGGCTTCCAGAGCGCTCTGGGTTCCGATGGGAGCCGGTGGTGGACACCGGCAAGGAGGCACCATATGACTTCCTCACCGATGGCCTGCCAGATCGTGCTGTCACCGTCTACCAGTTCTCTCATTTCCTCAACTCCAATCTCTATCCTATGCTCAGCTACTCCTCCATCATCCTTGTATTGCGCCCTGATGTCTGA Supplemental Figure S3: Map of the shuttle vector pMDC32 used for expression of the recombinant genes AtISA1-FL and and ZmISA1-FL into Arabidopsis thaliana. Arabidopsis (AtISA1-FL) and maize (ZmISA1-FL) full length cDNA sequences of ISA1 gene (depicted above) were introduced between the right (RB) and left (LB) borders of the modified T-DNA and placed under the control of the strong and constitutive 2x35S promoter. The NosT terminator sequence was placed downstream of ISA1 cDNA. KanR is the selection marker for E. coli and Agrobacterium tumefaciens conferring resistance to kanamycin. HygR is the selection gene that confers resistance to hygromycin B to the transformed plants.

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