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Gene structure DNA replication

Gene structure DNA replication. Figure 7.4A. Figure 7.5. The DNA double helix has grooves: a wide major groove and a narrow minor groove. - These provide DNA-binding proteins access to base sequences. DNA Structure. Figure 7.6. RNA differs from DNA: - Usually single-stranded

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Gene structure DNA replication

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  1. Gene structureDNA replication

  2. Figure 7.4A

  3. Figure 7.5

  4. The DNA double helix has grooves: a wide major groove and a narrow minor groove. - These provide DNA-binding proteins access to base sequences. DNA Structure Figure 7.6

  5. RNA differs from DNA: - Usually single-stranded - Contains ribose sugar - Uracil replaces thymine RNA Structure Figure 7.4B

  6. Bacteria pack their DNA into a series of loops or domains, collectively called the nucleoid. - Loops are anchored by histone-like proteins The Bacterial Nucleoid Figure 7.8

  7. DNA Replication Replication of cellular DNA in most casesis semiconservative. - Each daughter cell receives one parental and one newly synthesized strand. Figure 7.13

  8. Figure 7.14

  9. Figure 7.15

  10. Figure 7.18b Figure 7.18a

  11. Animation: DNA Replication DNA Replication Click box to launch animation

  12. Figure 7.19

  13. Figure 7.21

  14. Restriction endonucleases cleave DNA at specific recognition sites, which are usually 4 to 6 bp and palindromes. - May generate blunt or staggered ends Analysis of DNA Figure 7.27A

  15. Agarose gel electrophoresis can be used to analyze the DNA fragments obtained by treatment with restriction enzymes. Analysis of DNA Figure 7.27B

  16. The polymerase chain reaction (PCR)can produce over a million-fold amplification of target DNA within a few hours. Analysis of DNA Figure 7.29

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