1 / 12

Nucleotide Structure: Pentose sugar

Nucleotide Structure: Pentose sugar. Nucleotide Structure: Nitrogenous Bases. DNA Linkage. Phosphate group/phosphodiester bond Nucleotides contain one, two, or three phosphate groups. *nucleoside *nucleotide. Nucleic Acid Polarity. *Top of molecule has free 5’-phosphate group

braith
Download Presentation

Nucleotide Structure: Pentose sugar

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Nucleotide Structure: Pentose sugar

  2. Nucleotide Structure: Nitrogenous Bases

  3. DNA Linkage • Phosphate group/phosphodiester bond • Nucleotides contain one, two, or three phosphate groups *nucleoside *nucleotide

  4. Nucleic Acid Polarity *Top of molecule has free 5’-phosphate group This is the 5’ end *Bottom has a free 3’ hydroxyl group This is the 3’ end

  5. Structural analysis of DNA *Early 1950’s Rosalind Franklin *1953 Watson and Crick

  6. Complementarity

  7. DNA Structure:

  8. Brief Review of DNA Replication:

  9. Nearest Neighbor Analysis (Kornberg 1956): In vitro DNA synthesis •Template DNA •DNA polymerase •dNTPs *dATP *dCTP *dGTP *dTTP •Allow DNA replication to take place •Denature DNA •Treat with micrococcal nuclease- yields mononucleotides with 3’ terminal phosphates

  10. ssDNA treated with micrococcal nuclease: A A C C C * G G T T T T C ssDNA: *

  11. Exercise: List all possible dinucleotides (analysis of nearest 5’ neighbor) Which must be present in equal frequencies if the DNA strands are: Parallel ?Antiparallel?

More Related