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Nucleotide Substitutions (%)

Rhizopus oryzae. YR-2 - Brassica. EH-2 - Brassica. 100.0. CBG-3 - Brassica. 1. ZE-1 - Brassica. 100.0. NKa-3 - Brassica. GBR4 - Brassica. GBR5 - Brassica. 100.0. 2. 100.0. GBR7 - Brassica. GBR8 - Brassica. WT-1 - Lettuce. CH-1 - Tobacco. TAK-1 - Tobacco. KZ-1 - Lettuce. 3.

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Nucleotide Substitutions (%)

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  1. Rhizopus oryzae YR-2 - Brassica EH-2 - Brassica 100.0 CBG-3 - Brassica 1 ZE-1 - Brassica 100.0 NKa-3 - Brassica GBR4 - Brassica GBR5 - Brassica 100.0 2 100.0 GBR7 - Brassica GBR8 - Brassica WT-1 - Lettuce CH-1 - Tobacco TAK-1 - Tobacco KZ-1 - Lettuce 3 HY-1 - Lettuce NLD4 - Lettuce NLD3 - Lettuce ITA1 - Lettuce GBR10 - Cucumber 4 “Duke” - Melon 99.8 Y12830 WOms-3 – Welsh onion 97.8 AUS1 - Lettuce AUS2 - Lettuce BEL1 - Lettuce CHL1 - Lettuce DEU1 - Lettuce ESP1 - Lettuce ESP2 - Lettuce FRA5 - Lettuce GBR1 - Lettuce 5 GBR2 - Lettuce GBR3 - Carrot JPN1 - Lettuce NDL1 - Lettuce NDL2 - Lettuce PRT1 - Lettuce PRT2 - Lettuce USA2 - Lettuce 30.0 25 20 15 10 5 0 Nucleotide Substitutions (%)

  2. Fig. 2. Phylogram showing the relationship of Olpidium brassicae isolates from this study to each other and to isolates from the studies of Sasaya and Koganezawa (2006), James et al. (2006) [AFTOL 633] and Ward and Adams (1998) [Y12830], based on the sequence of the ITS1-5.8S-ITS2 region. The sequence of Rhizopus oryzae(GQ220706) was used as an out-group and bootstrap values greater than 95% (based on 1000 iterations) are shown adjacent to the appropriate branch points. Five distinct groups are indicated, with isolates from this study represented in four of the five (groups 2, 3, 4 and 5). The host plant species from which the isolates were obtained is shown next to the isolate designation.

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