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Project 3.2.2

Project 3.2.2. Using Chemical Indicators to Test the Presence of Macromolecules By: Nicolas Sbrocco. What Is a Chemical Indicator?.

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Project 3.2.2

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  1. Project 3.2.2 Using Chemical Indicators to Test the Presence of Macromolecules By: Nicolas Sbrocco

  2. What Is a Chemical Indicator? • Definition: A chemical indicator is a substance used to show visually usually by it’s capacity for color change, the condition of a solution with respect to the presence of free acid or alkali or some other substance. • Examples: Benedict’s solution, Iodine, Biuret solution, Sudan 4, Brown paper bag.

  3. How to Test for Monosaccharides • Procedures: • Fill test tube with 2 milliliters of water. • Add 2 milliliters of Benedict’s reagent to the solution • Heat test tube for 5 minutes • Record the color of the solution • Add glucose solution to the Benedict’s reagent and heat in boiling water for 5 minutes • Record the color of the solution • Positive Test: ORANGE • Negative Test: STAYS BLUE

  4. How to Test for Polysaccharides • Procedures: • Add 2 milliliters of iodine to 2 milliliters of water • Record color of solution • Add 2 milliliters of iodine to a starch solution • Record color of solution • Positive Test: DARK BLUE/BLACK • Negative Test: STAYS YELLOW

  5. How to Test for Proteins • Procedures: • Add 2 milliliters of Biuret reagent to 2 milliliters of water • Record color of the solution • Add 2 milliliters of Biuret reagent to a protein solution • Record color of the solution • Positive Test: LAVENDER/PURPLE • Negative Test: STAYS CLEAR

  6. How to Test for Lipids • Procedures: • Add 2 milliliters of Sudan 4 to 2 milliliters of water • Record the color of the solution • Add 2 milliliters of Sudan 4 to a oily/fatty solution • Record the color of the solution • Positive Test: RED • Negative Test: STAYS THE SAME

  7. Another Way to Test for Lipids • Procedure: • Obtain a brown paper bag • Put 2 drops of water on the bag and record what happens to the bag after 30 minutes • Put 2 drops of a fatty/oily solution on the bag and record what happens to the bag after 30 minutes • Positive Test: BAG BECOMES TRANSLUCENT • Negative Test: BAG STAYS THE SAME

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