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HPP – Shotgun Status

A.M. 30/11/2012. HPP – Shotgun Status. Project 22/8. FDR 1%: Protein level. Peptides & proteins by labs. Proteins by experiment. Exclusive Proteins ( including secretome ). Chr16 - distribution. Prot : 537 Genes: 385. Exclusive proteins Chr16.

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HPP – Shotgun Status

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  1. A.M. 30/11/2012 HPP – Shotgun Status

  2. Project 22/8

  3. FDR 1%: Proteinlevel

  4. Peptides & proteinsbylabs

  5. Proteinsbyexperiment

  6. Exclusive Proteins (includingsecretome)

  7. Chr16 - distribution Prot: 537 Genes: 385

  8. Exclusive proteins Chr16

  9. FDR 1% at peptidelevelfrom24 experiments

  10. FDR 1% at peptidelevelfrom 24 experiments

  11. Exclusive Proteins

  12. Exclusive Proteins of chromosome 16

  13. Proteinincreasewithexperimentnumber

  14. Quantitativeinformation? Onlyforchromosome 16 proteins Q = # peptides/kDa

  15. Quantitativeinformation? • 5 Lessabundantproteins of chromosome 16 • CHD9_HUMAN Chromodomain-helicase-DNA-bindingprotein • CBP_HUMAN CREB-bindingprotein • Isoform3 of Dynein heavy chain 3, axonemal • RBBP6_HUMAN Isoform 3 of E3 ubiquitin-protein ligase RBBP6 • ZN646_HUMAN Zinc fingerprotein646 • 5 Mostabundantproteins of chromosome 16 • RS2_HUMAN 40S ribosomalprotein S2 • RS15A_HUMAN 40S ribosomalprotein S15a • COTL1_HUMAN Coactosin-likeprotein • ELOB_HUMAN Transcriptionelongation factor B polypeptide 2 • ALDOA_HUMAN Fructose-bisphosphatealdolaseA

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