The Model Experiment

1. The Model Experiment

2. A Complex Behavior Modularize Module A Module B Module C Module D Behavior “orthologs” Well studied non-model example Find appropriate model system? Yes No Analyze Literature Search Mutants Literature Study Evaluate Candidate Genes Lab Assays Candidate Genes Experimental Methods Experimental Methods Generate Hypotheses and design experiments

3. Example Insect Behavior Module D Module A Module B Module C Ortholog 3 Ortholog 4 Ortholog 2 Ortholog 1 Fly, Mouse Fly Termite Fly Fly, Mouse Literature Mutants Literature Candidate Genes Lab Assays Experimental Methods Candidate Genes Experimental Methods Generate Hypotheses and design experiments

4. A Complex Behavior Analyze gene expression Variant A Variant B Variant C Variant D Gene expression profiles Ortholog analysis Analyze Look for Co-regulation Meta-analyze Study Functional annotation Correlated expression Pathways Generate Hypotheses and design experiments

5. Example Insect Behavior Microarray analysis Population B Population A Population C Genes with different expression profiles between populations Analyze Fly and mouse orthologs Look for Co-regulation Meta-analyze Study Functional annotation Correlated expression Pathways Generate Hypotheses and design experiments

6. The Fantastic BeeSpace Other entities Type query Entity Identifier Different PnPs Assay Engineer Behavior Candidate Gene Bank Mutant Base Module Maker Hypothesis Generator Tuned by user feedback Name and number the modules, describe each module Experiment Builder Ortholog Search Engine Model system Finder User select focal model system User approved