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The Eukaryotic Cell Fig 4.22, page 97. Eukaryotic cells have many unique features that distinguish them from prokaryotes Cilia Membrane sterols Ribosome structure Membrane bound organelles Nucleus. Structures External to Cell. Tubulin in 9+2 array with membrane covering Flagella
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Eukaryotic cells have many unique features that distinguish them from prokaryotes • Cilia • Membrane sterols • Ribosome structure • Membrane bound organelles • Nucleus
Structures External to Cell • Tubulin in 9+2 array with membrane covering • Flagella • Function in cell motility • long in relation to cell size • Cilia • Shorter; more numerous • Move cell or propel substances across cell
Glycocalyx • Carbohydrate coating in animal cells • Surrounds and stabilizes plasma membrane • Active in cell-cell recognition
Cell wall • Plants, algae, fungi • Cellulose, chitin, glucan, mannan, silica, calcium carbonate • NO PTG! • Penicillin a “miracle drug”? • Protozoa have no cell wall but some have a pellicle surrounding the plasma membrane
Eukaryotic Plasma Membrane • Similar in structure and function to prokaryote • Phospholipid bilayer embedded with proteins • Addition of carbohydrates and sterols (cholesterol, ergosterol) • Cell signaling • Cell recognition • Stability
Membrane proteins functions: • Transport • Maintain cell integrity • Attachment of proteins to internal structures • Receptors for cell signaling • Proteins in outer layer • Receptors typically glycoproteins
extracellular fluid (outside) binding site phospholipid bilayer carbohydrate cholesterol phospholipid receptor protein transport protein protein filaments recognition protein cytoplasm (inside)
Transport across eukaryotic membrane • Passive • Simple and facilitated diffusion; osmosis • Active • Active transport; endocytosis; exocytosis
Endocytosis • Bring in material from surrounding environment • Pinocytosis or Phagocytosis • Exocytosis • Releases contents into external environment
Cytoskeleton • Dynamic network of protein filaments (microtubules, actin filaments, intermediate filaments) • Anchor organelles, Structural support, Shape alteration, Movement
10 µm Column of tubulin dimers Tubulin dimer
Centrosome • Pair of centrioles and proteins found near nucleus • Involved in the assembly of microtubules
10 µm Actin subunit 7 nm
microtubules (red) nucleus microfilaments (blue)
Membrane-bound Organelles • Compartmentalization • Allows for division of labor • Nucleus • Mitochondria and chloroplast • Endoplasmic reticulum • Golgi apparatus • Lysosome and peroxisomes
Nucleus • nuclear envelope • Double membrane with pores • nucleolus • sites of rRNA synthesis • DNA typically in multiple pairs of linear chromosomes • associated with histones proteins
Sexual or asexual reproduction • mitosis and meiosis
Endoplasmic Reticulum • Network of flattened tubules • (cisterns) continuous with • nuclear membrane • rough ER • ribosomes present • Site of protein synthesis • smooth ER • no ribosomes • Site of lipid/fat synthesis
Ribosomes 80S Large 60S and small 40S subunits Membrane-bound: Attached to ER & nuclear membrane Free: In cytoplasm 70S In chloroplasts and mitochondria
Golgi Body • Curved cisterns • Not continuous • -Modifies, packages and secretes products of ER • - Produces lysosomes and vacuoles
Lysosomes • Formed in Golgi • Membrane enclosed sacs of digestive enzymes • Peroxisome • Oxidation of fatty acids; destroys H2O2 • Formed by dividing perosiomes • Vacuoles • Derived from the Golgi • Store nutrients • Sequester harmful substances
Mitochondria • Double membrane • - Inner membrane is folded into cristae • - Site of cellular respiration; ATP production • - 70S ribosomes and circular DNA; divide independently of cell Figure 4.26
Plastids • - Chloroplasts • - Found in algae and green plants • - Contains chlorophyll pigment • in the coin-like thylakoids • - Stacked thylakoids - grana • 70S ribosomes and circular DNA; • divide independently of cell Figure 4.27
Endosymbiotic Theory - Evolution from prokaryotes to eukaryotes 3.5 billion yrs ago - prokaryotes dominate 2.5 billion yrs ago - oxygen accumulates 1.5 billion yrs ago - 1st nucleated eukaryotes