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Introduction to the Eukaryotic Cell

Exercise 9. Introduction to the Eukaryotic Cell. Announcements. Post Lab 9/Pre Lab 10 are due by the time your lab meets next. LNA Eukaryotic Cell is due next week in lab. Goals. To learn the basic structures associated with eukaryotic cells.

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Introduction to the Eukaryotic Cell

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  1. Exercise 9 Introduction to the Eukaryotic Cell

  2. Announcements • Post Lab 9/Pre Lab 10 are due by the time your lab meets next. • LNA Eukaryotic Cell is due next week in lab.

  3. Goals • To learn the basic structures associated with eukaryotic cells. • To identify the differences between animal and plant cells. • To understand the basis of cell culture, and the changes imposed on cells under culture conditions. • To understand the techniques of fluorescence and electron microscopy and cell culture.

  4. Animal Cell

  5. Plant Cell

  6. The Exercise • Part I: Plant cells: Onion epidermis • Basic cell structure of a plant lacking chloroplasts • Iodine: observe nucleolus • Janus Green: observe mitochondria

  7. The Exercise • Part II: Animal cells: Human squamous epithelium • Observe differences from the plant cells • Do the mitochondria vary in size from those found in plant cells?

  8. The Exercise • Part III: Autofluorescence • View Tradescantia under the fluorescent microscope • Chlorophyll pigments autofluoresce

  9. The Exercise • Part IV: Anacharis Leaf Cells • View a living plant cell with chloroplasts

  10. The Exercise • Part V: Electron Micrographs and Cellular Infrastructure • View EM transparencies • Identify organelles • You may want to take notes for future identification (i.e. Exam 2 and Final)

  11. Be Able to Identify: Nucleus Nuclear envelope Nuclear pore Nucleolus Rough Endoplasmic Reticulum Ribosome Mitochondria

  12. Be Able to Identify: Golgi complex top view section view

  13. Golgi top view

  14. Golgi section view

  15. Be Able to Identify: Mitochondrion

  16. Be Able to Identify: Nucleus Vacuole Chloroplast

  17. Be Able to Identify: Thylakoid

  18. Summary of Exercise 9 • Part I: Onion epidermis • slide x 3: water / Iodine / Janus Green • size, nucleus, vacuole, cell wall/membrane, chloroplast, mitochondria (?) • Part II: Cheek cells • slide x 2: water / Janus Green • size, nucleus, cell membrane, mitochondria (?) • Part III: Tradescantia • TA • Autofluorescence • Part IV: Anacharis • Cytoplasmic streaming

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