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Summary of the current status of the work of TUM-BO

Summary of the current status of the work of TUM-BO. Andreas Gattinger, Matthias Weiss, Michael Schloter GSF-Research Center for Environment and Health Technical University of Munich Institute of Soil Ecology D- 85764 Neuherberg. Summary of the current status of the work of TUM-BO.

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Summary of the current status of the work of TUM-BO

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  1. Summary of the current status of the work of TUM-BO Andreas Gattinger, Matthias Weiss, Michael Schloter GSF-Research Center for Environment and Health Technical University of MunichInstitute of Soil Ecology D- 85764 Neuherberg

  2. Summary of the current status of the work of TUM-BO 1. Adaptation of protocols for lipid biomarker analyses to peat (bog) samples 2. Adaptation of protocols for DNA analyses to peat (bog) samples 3. Growing of keystone plant species in the greenhouse for plant litter labelling 4. Installation of the new analytical device GC/MS-C-IRMS for compound specific isotope analysis

  3. 1. Adaptation of protocols for lipid biomarker analyses to peat (bog) samples

  4. genotypic approach:(example archaeal 16S rDNA, separation with SSCP ) phenotypic approach:(side chain analysis of polar membrane lipids) 1 PLEL derived hydrocarbons 8 6 3 10 5 9 12 11 2 7 13 4 14 15 0 Determination of microbial communites • Problem: less than 5% of the estimated total soil microflora can be cultivated • Solution:cultivation-independent approaches enable the characterization of microbial communiteis in situ:

  5. The biosphere of soil Archaeal kingdoms (Reiche): - Euryarchaeota - Crenarchaeota - (Korarchaeota) - (Nanoarchaeota) methanogens (mod. Version, M. Schmid, GSF)

  6. Side chain analysis of polar membrane lipids of BacteriaEucarya and Archaeain soil Bacteria & Eucarya esterlinked fatty acids (PLFA):- saturated (SATFA)- monounsaturated (MUFA) - polyunsaturated (PUFA) - hydroxysubstituted (PLOH) Archaea etherlinked isoprenoids (PLEL):- saturated short chain: i20:0 - saturated long chain: i40:0 - cyclic long chain: i40:0-cy- unsaturated short chain: i20:1 Bacteria & Eucarya non-esterlinked fatty acids (NEL-PLFA):- hydroxysubstituted (UNOH) - unsubstituted fatty acids (UNSFA)

  7. Extended phospholipid analysis x Bodenökologie

  8. Microbial biomarker composition in a Scottish “test” peat core

  9. Lipid biomarker ratios in a Scottish “test” peat core

  10. MOB and archaeal biomarker in a Scottish “test” peat core

  11. 2. Adaptation of protocols for DNA analyses to peat (bog) samples together with Rebekka Artz, Steve Chapman/MLURI

  12. 3. Growing of keystone plant species in the greenhouse for plant litter labelling

  13. Plants were taken by A. Siegenthaler et al. at the French/Suisse site: 4 plant species: 2 mosses (Sphagnum, Polytrichum), 2 grasses (Eriophorum vaginatum, Eriophorum angustifolium) were grown at the GSF greenhouse for estimating growth conditions and biomass production climatic conditions:

  14. 4. Installation of the new analytical device GC/MS-C-IRMS for compound specific isotope analysis

  15. Simultaneous identification and quantification of PLFA/PLEL from environmental samples and their corresponding 12C/13C ratios by GC/MS-C-IRMS 20% of the analyte MS(DSQ) IRMS(DeltaPlusAdvantage) 80% of the analyte

  16. delta 13C values in PLFA/PLEL from test core samples varied between -26 and -32 %0 (natural abundance): due to dilution effects with the peat soil and 1-2% measurement inaccuracy, an enrichment with 13C in the plant litter of at least 70 %0 PDB is needed: Which quality of CO2 gas for plant labelling?

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