1 / 15

EXERCISE, LIPIDS AND ANTIOXIDANT STATUS IN TYPE 2 DIABETES MELLITUS

EXERCISE, LIPIDS AND ANTIOXIDANT STATUS IN TYPE 2 DIABETES MELLITUS. Kostic N, Caparevic Z, Ilic S, Dekleva M, Pencic B, Popovic S. Endocrinology department, CHC Dr Dragisa Misovic - University of Medicine Belgrade, Serbia and Montenegro. Introduction.

calliope
Download Presentation

EXERCISE, LIPIDS AND ANTIOXIDANT STATUS IN TYPE 2 DIABETES MELLITUS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. EXERCISE, LIPIDS AND ANTIOXIDANT STATUS IN TYPE 2 DIABETES MELLITUS Kostic N, Caparevic Z, Ilic S, Dekleva M, Pencic B, Popovic S. Endocrinology department, CHC Dr Dragisa Misovic - University of MedicineBelgrade, Serbia and Montenegro

  2. Introduction • We hypothesize that a physical training program induces an enhance on muscular and liver antioxidant enzymes and the reduction of oxidative stress.

  3. Aim • Our aim was to evaluate oxidative stress and antioxidant parameters in 50 type 2 diabetic patients; with or without complications and also to determine the effect of acute stress exercise on the investigated parameters. • We also wanted to determine if the action of acute physical exercise on antioxidant parameters is the different for the patients with diabetes mellitus type 2 compared with the controls. • Within the diabetic group, we investigated the influence of complications by measuring pre- and postexercise levels of some byochemicals and antioxidant parameters.

  4. Methods-subjects • Patients with diabetes were divided into groups using several criteriums such as: the presence of diabetic microangiopatic complications(29 without complications and 21 with complications). None of the patients had a history of cerebrovascular or ischemic heart disease.They were not treated with any other drugs except oral antidiabetics. • For providing an objective assesment of exercise capacity and impairment we applied cardiopulmonaly exercise cycle ergometar test (Jaeger Oxycon Delta ER-900).

  5. Methods-biochemicalmeasurements • Lipids profiles were measured by enzymatic methods. • Oxidized LDL-cholesterol was measured by Elisa method (Mercodia). • HbA1c was measured by immunoturbidometric method using commercialy available kit (Roche). Hitachi 902 analyser system was used. • Glucose were determined by enzymatic methods. • Plasminogen activator inhibitor/tip 1 (PAI -1) was measured by spectrophotometric method using commercialy kit (Behring).

  6. Methods-antioxidant parameters • The activity of superoxide dismutase (SOD) was measured at 500 nm with a commercialy available kit (Randox Laboratories,kit Ransod superoxide dismutase) by testing the inhibition degree of a tetrazolium salt oxidation reaction. The coefficient of variability between assays was 4,2%. • The erythrocyte activity of Glutathione peroxidase (GSH-Px U/g Hb) was evaluated with a commercialy kit (Ransel glutathione peroxidase,Randox Laboratories) in erythrocytes at 340 nm by measuring the decrease of NADPH absobance.The coefficient of variability between assays was 4%.

  7. Results • We will first compare the diabetic patients group to those of the control group, then we will compare results within two diabetic subgroups (with and without complications).

  8. Results-Clinical and biochemical parameters in diabetic patients *p<0,01 ; ‘p<0,05

  9. Results-Biochemical and antioxidant parameters of diabetics with and without complications before and after exercise test *p<0,01 ; ‘p<0,05

  10. Results-Respiratory and Cardiovascular Postexercise Data for Healthy and Diabetic Groups *p<0,01 ; ‘p<0,05

  11. Results- • Our results showed that after the acute exercise, Ox-LDL was significantly increased only in type 2 diabetic patients without complications (p<0,01). • GSH-Px was significantly increase only in diabetic patients (with and without complications) after acute exercise (p<0,01) but not in controls. • PAI-1 significantly decresed during the exercise test, only in healthy subjects (p<0,05).

  12. Results- GSH-Px was significantly increase only in diabetic patients after acute exercise (p<0,01) but not in controls.

  13. Results • There was a negative correlation between 1) GSH-Px and TG (r = -0,5899,p <0,05) 2) GSH-Px and PAI-1(r = -0,5337, p<0,05) 3)VO2 peak and Ox LDL before exercise (r= -0,65, p< 0,05) 4) OHR and Ox LDL after exercise (r= -0,697, p< 0,05)

  14. Results Correlation analysis revealed a significant negative correlation (during exercise) between GSH-Px and glycemia (r = -0,6336, p<0,01)

  15. Conclusion • The evidence of a high oxidative profile during exercise in diabetes type 2 has not been directly related to an increase risk of CVD. • We summarized that acute exercise induces a sudden increase of oxidative stress levels, an effect well counterbalanced by both the type 2 diabetic patients and in the healthy subject. • We suggest that the greater concentrations of Ox LDL, and GSH-Px activities after exercise test in type 2 diabetic patients could be a compensatory mechanism by the body to prevent tissue damage. • After a physical training program an improvement on the coumterbalance of the oxidative stress could be expected.

More Related