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3D reconstruction from confocal microscopy data

3D reconstruction from confocal microscopy data. Alex Sossick Wellcome/CRC Institute University of Cambridge. A brief history. Interest in magnification has been around for a long time, 2nd Century BC: Claudius Ptolemy:

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3D reconstruction from confocal microscopy data

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  1. 3D reconstruction from confocalmicroscopy data Alex Sossick Wellcome/CRC Institute University of Cambridge

  2. A brief history • Interest in magnification has been around for a long time, • 2nd Century BC: Claudius Ptolemy: • Described a stick appearing to bend in a pool of water, and accurately recorded the angles to within 1/2 degree for this relationship. He then very accurately calculated the refraction constant of water. • 1st Century AD: Seneca: • Described actual magnification by a globe of water. He wrote the following: "Letters, however small and indistinct, are seen enlarged and more clearly through a globe of glass filled with water." • Specticals were reinvented circa 1280-1285, • (the Chinese already ‘cosmetically’ had invented specticals by along time before this)

  3. A brief history • Credit for the first microscope is usually given to Zacharias Jansen, in Middleburg, Holland, around the year 1595. • By the 1880’s the theoretical resolving power was achieved, 2 points only 0.2 microns apart. • First production confocal late 1980’s

  4. What is Confocal Microscopy? • It is similar to conventional epi-illumination light microscopy. • The confocal pinhole removes out of focus light, therefore optical sectioning is possible. • The main advantage is the ability to optical section through a sample. • It is non-invasive

  5. A Confocal Microscopy

  6. Nipkow Confocal

  7. Nipkow Confocal

  8. Why 3D reconstruction? • Alot more structural information can be gained from looking at a sample at different view points.

  9. AVS/EXPRESS • AVS/Express gives a flexible approach to 3D image processing

  10. The Video!!! A Wellcome/CRC production

  11. The future with AVS/Express • Better 3D Imaging • Segmentation • 4D Image processing • Projections through time • Slices through time

  12. A function of Z through time

  13. The people who did the work! Andrea Brand Jim Hasloff Emma Dormand Isabel Palacios

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