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Stable Isotope and Metabolomics Core Facility

Stable Isotope and Metabolomics Core Facility.

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Stable Isotope and Metabolomics Core Facility

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  1. Stable Isotope and Metabolomics Core Facility The Einstein Stable Isotope & Metabolomics Core is within the NIH funded Diabetes Research Center at Einstein and anchors the CMCR Metabolomics Core. The Core uses stable isotope flux and metabolite profiling to help formulate and test hypotheses about the metabolic consequences of various changes in gene expression and protein function, in order to guide further integrative systems biology analyses of the underlying mechanisms in diabetes, insulin resistance, obesity, and diabetic complications. The Core objectives includes assessments of plasma, urine and tissue metabolite profiles for diagnosis/characterization of physiological and pathophysiological states. These include key metabolites in the glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic cycle pathways, fatty acid oxidation and complex lipids, such as ceramides, diacylgylcerols, triacylglycerols (TAGs), and phospholipids. In the CMCR framework, the Core will use these methodologies to explore the specific effects that radiation has on the metabolic network of each organ, and to explore the metabolic consequences of protein modification, by radiation, on the function of the metabolic network. AECOM Diabetes Center

  2. Albert Einstein College of Medicine OF YESHIVA UNIVERSITY CMCR-AECOM Stable Isotope & Metabolomics Core Location: Price 368 Director: Irwin Kurland, MD PhD Lab Members: Sandhya Narasimhan, PhD Bhavapriya Vaitheesvaran, PhD Seahorse Extracellular Flux Analyses Metabolite Profiling and Flux Analyses • Kirsten Hartil, PhD Mitochondrial Physiology Animal Research Coordinator • Hui-Min Zhang PhD LC/MS Specialist

  3. XF Applications in Cellular Bioenergetic Assays Obesity/diabetes Cardiovascular research XF96 Extracellular Flux Analyzer Mitochondrial Function Neurodegenerative diseases Fatty Acid Oxidation & Glycolysis Translation to Clinical Applications Cancer Cell quality In vitro Toxicity

  4. Waters Aquity High resolution Chromatography LC/MS Analysis Simultaneous analysis of multiple metabolites Multivariate Statistical Analyses Identification of unknown species by structure elucidation LTQ-Orbitrap

  5. Waters Xevo Tandem MS

  6. Synapt G2 Linear Hybrid quadrapole Time of Flight MS with MALDI Synapt G2 itself Synapt G2 with MALDI front end

  7. GC/MS Analysis of Metabolites Metabolites from the glycolytic pathway, TCA cycle, pentose cycle and amino acids Lipid profiling: Fatty acids DAGs, TAGs Cholesterol Stable isotope assessments of dynamic glucose disposal (SipGTT), lipogenesis ([2H]2O or U-13C]lactate), glucose production ([U-13C]glucose), glycerol production/lipolysis ([2-13C]glycerol), Cori cycle ([U-13C]lactate)

  8. Waters Premier GC-Time of Flight-MS

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