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RNA Polymerase binding. *Constitutive or basal level transcription. Allosteric activation of RNA polymerase. Inhibition of transcription. DNA-protein interactions. Negative Control of the lac Operon. Proposed CAP-cAMP Activation of lac Transcription. Transcription in Eukaryotes.
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RNA Polymerase binding *Constitutive or basal level transcription
Inhibition of transcription DNA-protein interactions
Transcription in Eukaryotes Three major classes of RNA: Type Encodes Copies/genome % Total RNA mRNA proteins Single or few 10% tRNA functional RNA Few 20% rRNA functional RNA Many 70% But there are more!!! S.R. Eddy (2001) Noncoding RNA genes and the modern RNA world, Nature Reviews Genetics, 2:919-929
Epitope Tagging of RNA Polymerase II *Genetically “tag” Polymerase II subunit *Grow cells in radioactively labeled amino acids *Allow assembly of active polymerase *Add antibody that binds epitope *”Grab” antibody and what ever it is bound to *SDS electrophoresis
Transcription *RNA polymerase II
Transcription *RNA polymerase II