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RNA Polymerase binding

RNA Polymerase binding. *Constitutive or basal level transcription. Allosteric activation of RNA polymerase. Inhibition of transcription. DNA-protein interactions. Negative Control of the lac Operon. Proposed CAP-cAMP Activation of lac Transcription. Transcription in Eukaryotes.

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RNA Polymerase binding

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  1. RNA Polymerase binding *Constitutive or basal level transcription

  2. Allosteric activation of RNA polymerase

  3. Inhibition of transcription DNA-protein interactions

  4. Negative Control of the lac Operon

  5. Proposed CAP-cAMP Activation of lac Transcription

  6. Transcription in Eukaryotes Three major classes of RNA: Type Encodes Copies/genome % Total RNA mRNA proteins Single or few 10% tRNA functional RNA Few 20% rRNA functional RNA Many 70% But there are more!!! S.R. Eddy (2001) Noncoding RNA genes and the modern RNA world, Nature Reviews Genetics, 2:919-929

  7. Epitope Tagging of RNA Polymerase II *Genetically “tag” Polymerase II subunit *Grow cells in radioactively labeled amino acids *Allow assembly of active polymerase *Add antibody that binds epitope *”Grab” antibody and what ever it is bound to *SDS electrophoresis

  8. Transcription *RNA polymerase II

  9. Transcription *RNA polymerase II

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