Working with Animals in the Hood or Change Station

1. Working with Animals in the Hood or Change Station Patricia Matos Julie Watson

2. THE CAGE IS THE PRIMARY BARRIERThe barrier to disease is at the CAGE level. If the cage is closed, the cage protects. EVERY TIME you open the cage the animals are at risk ONLY open the cage in a disinfected hood or change station

3. THE HOOD PROVIDES AN AIR BARRIERWhen you open the cage, the cage barrier is lost. Instead, the hood & change station protect with a filtered air curtain NEVER block the air vents NEVER put your head or body in the hood or change station. You contaminate the space and disrupt the air barrier

4. THE AIR CURTAIN PROTECTS YOUR ANIMALS The protected area is WITHIN the air vents, providing the face screen is in the correct position ALWAYS work in the protected space inside the air vents ALWAYS keep the side panels DOWN when working in the change station

5. YOU MUST MAKE SURE THE HOOD IS CLEAN The hood & change station may harbor pathogens from a prior user First spray all surfaces with disinfectant (MB-10) Then wipe down to make sure all areas are covered with disinfectant

6. PREPARE IN ADVANCEGather all your materials in advance to avoid trips away NEVER leave your hood unattended. How do you know it will not be compromised while you’re gone?

7. USE THE RIGHT PROTECTIVE EQUIPMENTAlways use disinfected gloves and sleeves Don’t leave a gap between gloves and sleeves. Use tape or poke thumb through sleeve. Cover sleeve with glove. Spray gloves and sleeves with disinfectant (MB-10)

8. ORGANIZE YOUR WORK AREAMessy space leads to mistakes Define your animal work area. You may use a clean towel or diaper If using a towel,it can spread pathogens. Replace towel between cages to avoid spreading disease.

9. THE OUTSIDE OF THE CAGE MAY HARBOR PATHOGENS Don’t put those pathogens in the hood! Spray OUTSIDE of cage with MB-10 BEFORE placing it in the hood!

10. WORK WET. KEEP USING THE DISINFECTANTDon’t spread infection from outside the cage to the animals inside ALWAYS wet hands in MB-10 after handling outside surfaces, cage or cage lid ALWAYS dip instruments and wet hands in MB-10 between cages

11. DISEASE ALSO SPREADS BY AEROSOLIf more than one cage is open, they are sharing both airspace and disease Open only the cages you have to. Close cages as soon as you are finished. Don’t overload the hood. Replace cages on rack once finished.

12. YOU MUST ENSUREYOUR CAGES HAVE FOOD AND WATER When returning cages to the rack: ALWAYS touch the water valve to get water flow ALWAYS lock cages in to ensure air flow.

13. LEAVE THE HOOD AS YOU WOULD WISH TO FIND IT Remove all equipment Spray down and wipe hood surfaces Discard trash Remember: report problems to supervisor immediately

14. SUMMARY 1. Use gloves and sleeves all Walls 2. Soak hood surfaces Walls Walls Ceiling outside Floor of cage 3. Spray wet ! 4. Work