1 / 8

arNOX, AGING-RELATED CELL SURFACE ECTO-NOX PROTEIN

arNOX, AGING-RELATED CELL SURFACE ECTO-NOX PROTEIN. D. James Morr é and Dorothy Morr é Department of Medicinal Chemistry & Molecular Pharmacology and Department of Foods and Nutrition Purdue University, W. Lafayette, Indiana 47907. Preventative target to reduce atherogenic risk in the elderly.

carson
Download Presentation

arNOX, AGING-RELATED CELL SURFACE ECTO-NOX PROTEIN

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. arNOX, AGING-RELATED CELL SURFACE ECTO-NOX PROTEIN D. James Morré and Dorothy Morré Department of Medicinal Chemistry & Molecular Pharmacology and Department of Foods and Nutrition Purdue University, W. Lafayette, Indiana 47907

  2. Preventative target to reduceatherogenic risk in the elderly

  3. 1/2 O2 H2O - 2 O2 NOX 2 O2 S SH Protein Protein Outside S SH Plasma Membrane Q10 Q10H2 Inside Quinone Reductase NAD(P)+ NAD(P)H + H+

  4. Glucose Glucose ADP NAD + NAD+ + A Glycolysis Glycolysis ATP PMOR NADH NADH AH + H+ + + H+ 2 Pyruvate Pyruvate Anaerobic Mitochondrial ATP Production Enhanced PMOR Activity Cell DNA mutations by Glycolysis to Oxidize NADH Adjacent cells LDL - O 2 1/2 O 2 2 H+ + Atherosclerosis 2 e- - H O 2 . oxLDL H O 2 2 OH• Generation of Propagation ROS at PM of Response

  5. SOD – inhibited reduction of ferricytochrome c Human (Age > 50y) • Buffy coats • Sera • Saliva • Perspiration Late-passage cell culture • MCF-10A • WI-38 Plants • Senescent spinach leaves • Senescent Aracean spathes

  6. Morré, D.M, F. Guo and Morré, D.J. 2003 Mol. Cell. Biochem. 254:101-109.

  7. Ar NOX inhibited by • Coenzyme Q (Q8, Q9 and Q10 but not by Q0, Q2, Q4, or Q7) • Several Chinese botanicals with antiaging activities Buffy Coats Morré, D.M and Morré, D.J. 2003 BioFactors 18:33-43

  8. Summary Aging-related NOX • Generates superoxide • Cells and sera of aged individual (>50y) • Postulated to propagate aging cascade to adjacent cells and circulating lipoproteins • Ablated by coenzyme Q10 /Antiaging botanicals

More Related