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Study of protein production patterns in biofilms observed through 2D images at different maturation stages. Identification and labeling of trend I, II, and III proteins displaying distinct patterns. Comparative analysis for understanding protein distribution.
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Pattern A Supplement 1a. Locations of trend I proteins displaying the distinct pattern A. 2D-images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns are indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.
Pattern B Supplement 1b. Locations of trend I proteins displaying the distinct pattern B. 2D-images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.
Pattern C Supplement 1c. Locations of maturation-1 stage specific proteins (trend I, pattern C). 2D-images were obtained from maturation-1 stage biofilms (3 days). Labeled proteins indicate the location of proteins displaying the protein production pattern C. The proteins were found to be maturation-1 stage-specific. The image on the right represents the same 2D-image without the labels.
Pattern D Supplement 1d. Locations of trend I proteins displaying the distinct pattern D. 2D-images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.
Pattern E Supplement 2a. Locations of trend II proteins displaying the distinct pattern E. 2D-images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.
Pattern F Supplement 2b. Locations of trend II proteins displaying the distinct pattern F. 2D-images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.
Pattern G Supplement 3. Locations of trend III proteins displaying the distinct pattern G. 2D-images were obtained from late-stage, dispersion biofilms (9 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.