1 / 28

Buck Scientific, Inc .

Buck Scientific, Inc. Seminar in ANALYTICAL INSTRUMENTATION for Spectroscopy & Chromatography: UV-Vis Spectrophotometry. Ultra-Violet/Visible SPECTROSCOPY (UV-Vis). Theory & Principle of OPERATION.

casey-guy
Download Presentation

Buck Scientific, Inc .

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Buck Scientific, Inc. Seminar in ANALYTICAL INSTRUMENTATION for Spectroscopy & Chromatography: UV-Vis Spectrophotometry

  2. Ultra-Violet/Visible SPECTROSCOPY (UV-Vis) Theory & Principle of OPERATION • A TYPE of VIBRATIONAL Spectroscopy, similar to Infra-Red. A Sample in a Cuvette is exposed to Light Energy between 190nm. (in the UV) and 1000nm. (in the VIS). Certain parts of an Organic Molecule will ABSORB some of this energy to create PEAKS on a SPECTRUM for Quantitative (primarily) and Qualitative Analysis

  3. BASIC Spectrophotometer Design Like AAS instrumentation, the ORIGINAL UV-Vis Specs were made as DOUBLE-BEAM units to correct for Noise, Drift and other Instabilities. Over 20 years ago, the well-known leaders in the Analytical Instrument markets; Beckman & Perkin-Elmer; began to focus on a line of “Stable-Beam” SINGLE-BEAM Instruments. The BUCK/Cecil series employs this optimum design in our instruments with unique Internal software

  4. Basic SPECTROMETER Design • ALL Analytical Spectrometers have several BASIC design components in common: • Light Source • Sample “Chamber” • Optical System • Detector • Output

  5. Basic SPECTROMETER Design • ALL Analytical Spectrometers have several BASIC design components in common: • LIGHT SOURCE: External D2 & W Lamps to prevent • thermal instability within instrument (no fan) • SAMPLE “Chamber”: Standard & Specialty Holders • OPTICS: High-energy Ebert Monochromator • DETECTOR: Choice of low-Noise Photo-Diode or high-Sensitivity Photo-Multiplier Tube • OUTPUT: Graphics On-Screen Display, Analog, Printer • and Serial RS-232 P-C capabilities

  6. UV/Vis Important Features • Scan Speed • Resolution • Software Features • Ease of Operation • Data Storage • Customized Calculations • Support & Service

  7. BUCK/Cecil Instruments • The COMPACT, “Stable-Beam” construction of the Single-Beam series uses the high-quality Cecil base instrument and high-performance components from BUCK Scientific

  8. BUCK/Cecil Instruments • Best optical design and highest quality components resulting in superior specifications: • Stray Light • Noise • Stability • Baseline Flatness • Accuracy (wavelength & photometric / ABS) • Reproducibility (wavelength & photometric / ABS)

  9. BUCK/Cecil Series • Series 2000 • Single Beam, Scanning, 4nm bandpass • Optional built-in printer & P-C Interface • Many software options • Most POPULAR Industrial system • Applications: Bio-Quest, Aqua-Quest, Food-Quest series with Prep-Programmed Operations

  10. BUCK/Cecil Series • Series 3000 • Single Beam, Scanning, 1.8nm bandpass • Optional built-in printer & P-C interface • Many software features (Kinetics, Bio-Assay) • Reflectance option: 3055 w/ Integrating Sphere Specifically designed to meet ALL of the required Specifications for USP / BP Pharmaceutical Assays

  11. BUCK/Cecil Series • Series 7000/7000+/9000 • Double-Beam, Scanning, some Models with • variable 0.5 / 1.0 / 1.8-2.0 / 4.0nm bandpass • Standard Printer & P-C interface, Data-Stream • Many software features (Kinetics, Bio-Assay) • Reflectance / Fluorescence / Turbidity options Ideal for the stringent requirements of Academic and Pharmaceutical Research

  12. Sample Handling Options • Cuvette - standard cell holder • Quartz for UV range • Glass & Plastic for Vis range • 1-100mm Pathlengths • Flow Cell - standard or micro flow (50L) • Uses Sipette Pump • Optional Automatic Cell Changer

  13. Sample Handling Options • Multi-Cell Changer • 4, 6, or 8 Positions • Manual, Automatic, Programmable • Thermostatted Cell Holder • Water-jacketted or Electronic Peltier system • Batch Sampler - 40 Positions Carousel • Specular & Integrating Sphere / Diffuse Reflectance

  14. Sample Handling Options Multiple position CELL CHANGERS, MICRO-SIPPERS and Sequential Access BATCH SAMPLERS Allow the Chemist complete FLEXIBILITY for MAXIMUM Sample Throughput & Volume on ANY of the BUCK/Cecil UV-Vis Spectrophotometers

  15. Software Features A convenient INTERNAL Ink-Printer, and several P-C Software Programs provide many Data Processing and Manipulation options for Qualitative & Quantitative Work Economical UV-Scan and sophisticated GRAMS/UV & Data-Stream are available

  16. Software Features The INTERNAL Software built-in to the BUCK/Cecil UV-Vis Spectrophotometers is capable of performing General Analytical, Environmental, Biotechnology and Clinical Assay WITHOUT the need for a Computer: • Christian-Warburg & Protein / DNA Measurements • Colorimetric Anion Calibrations stored in the instrument • Wavelength Subtraction, Spectral Overlay & Smoothing, • Derivatization and auto Peak-Pick on all systems • Multi-Component Analysis, with 1o / 2o and 3o Polynomial • Calibrations with up to 10 Standards with Statistics

  17. APPLICATIONS • Primarily used for QUANTITATIVE Analysis of Known Compounds, UV-Vis is one of the most popular techniques in the Pharmaceutical, Foods and Paints Industries, as well as Water Laboratories

  18. APPNOTE - Inorganic • SAMPLE:River Water for PHOSPHATE • PREPARATION: Digest w/ 10% HCl, add NH4MoO4 & Hydrazine reagent, boil till Blue color develops; measure at 810nm. • ANALYSIS: CE-2041 or VIS-100 w/ USEPA 2-pt Calibration RESULTS: mg/L or PPM in Sample

  19. APPNOTE - Inorganic • DATE Total-PO4 ortho-PO4 • 3-10-00 69 PPM 51 PPM • 3-24-00 77 PPM 50 PPM • 4-08-00 94 PPM 83 PPM • 4-22-00 118 PPM 72 PPM • 5-10-00 85 PPM 78 PPM • ANION assays by Colorimetry are the ACCEPTED Standard World-Wide & much better (and less expensive) than Ion-Chromatography

  20. APPNOTE - Cosmetic • SAMPLE:Color-Dyes for Hair Shampoos • PREPARATION: Make 1:5 and 1:200 dilution of Dyes to compare to Samples • ANALYSIS: CE-2041 or -3041 Scanning Spectrophotometer, • 1cm. Glass Cuvette RESULT: % Red / Green / Blue based on Color Standards

  21. APPNOTE - Nutritional • SAMPLE: Cereals and Grains (preserved) • PREPARATION: Hydrolyze 2 grams w/ 5% HCl, extract w/ Hexane for 1:10 dilution • ANALYSIS: CE-2041 or CE-3041 Scanning • Spectrophotometer, scan range 200-450nm. For BHT, BHA and TBHQ Anti-Oxidants. Sample solutions run in 10mm. Quartz Cells RESULT: Parts-Per-Million TOTAL Anti-Oxidants based on USFDA Standard Procedure

  22. APPNOTE - Nutritional • Sample BHA BHT TBHQ • [Wavelength 410nm. 360nm. 285nm.] • Frosted Flakes 35 PPM 27 PPM 11 PPM • Raisin Bran 16 PPM 41 PPM < 2 PPM • Rice Krispies 23 PPM 11 PPM 4.5 PPM • Scanning SPECTROPHOTOMETRY is an ideal • method to evaluate YOUR OWN products, or material from the Competition

  23. Other SPECTRO Analyzers from BUCK • BUCK Scientific also offers a series of inexpensive, high-performing and reliable Analytical Spectroscopy equipment for special Applications, Educational and Dedicated Testing procedures

  24. FLUORESCENCE SPECTROSCOPY (FLUOR) Theory & Principle of OPERATION • FLUORESCENCE is a form of Molecular “EMISSION” Spectroscopy; where a Sample in a special Cuvette is exposed to invisible, high-Energy UV radiation, and certain parts of the Organic Molecule will “give off” or EMIT lower-Energy VISIBLE Light radiation. • Very specific & selective test for Drugs & Vitamins, Alkaloids & Toxins

  25. BUCK Filter Fluorometer • The advantage of HIGH Sensitivity for most compatible materials makes Fluorescence a valuable analytical tool for Forensic & Drug applications Intense BLUE Fluorescence of QUININE in a Drug Formulation

  26. BUCK Filter Fluorometer • The availability of a wide assortment of Excitation and Emission Filters makes this portable FL-380 instrument very flexibility for a very low cost… ideal for Academic Teaching & Industrial QA/QC work

  27. Visible COLORIMETRY (COLOR) Theory & Principle of OPERATION • This is for use in Academic Teaching classes and for dedicated COLORIMETRIC Analyses for materials such as ANIONS (SO4, NO2, NO3, PO4, etc) and Visible Colors of compound such as Pigments, Chlorophyll and Dyes • Covers the range from 320 - 950nm.

  28. BUCK Colorimeter • The VIS-100 is a small, durable, self-calibrating unit that provides MEDIUM resolution @ 8nm. Bandpass, automatic 1-point Calibration & Data Outputs for a very low price

More Related