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Buck Scientific, Inc.

Ultra-Violet/Visible SPECTROSCOPY (UV-Vis). A TYPE of VIBRATIONAL Spectroscopy, similar to Infra-Red. A Sample in a Cuvette is exposed to Light Energy between 190nm. (in the UV) and 1000nm. (in the VIS). Certain parts of an Organic Molecule will ABSORB some of this energy to create PEAKS on a SPEC

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Buck Scientific, Inc.

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    1. Buck Scientific, Inc. Seminar in ANALYTICAL INSTRUMENTATION for Spectroscopy & Chromatography: UV-Vis Spectrophotometry

    2. Ultra-Violet/Visible SPECTROSCOPY (UV-Vis) A TYPE of VIBRATIONAL Spectroscopy, similar to Infra-Red. A Sample in a Cuvette is exposed to Light Energy between 190nm. (in the UV) and 1000nm. (in the VIS). Certain parts of an Organic Molecule will ABSORB some of this energy to create PEAKS on a SPECTRUM for Quantitative (primarily) and Qualitative Analysis

    3. BASIC Spectrophotometer Design

    4. Basic SPECTROMETER Design ALL Analytical Spectrometers have several BASIC design components in common: Light Source Sample “Chamber” Optical System Detector Output

    5. Basic SPECTROMETER Design ALL Analytical Spectrometers have several BASIC design components in common: LIGHT SOURCE: External D2 & W Lamps to prevent thermal instability within instrument (no fan) SAMPLE “Chamber”: Standard & Specialty Holders OPTICS: High-energy Ebert Monochromator DETECTOR: Choice of low-Noise Photo-Diode or high-Sensitivity Photo-Multiplier Tube OUTPUT: Graphics On-Screen Display, Analog, Printer and Serial RS-232 P-C capabilities

    6. UV/Vis Important Features Scan Speed Resolution Software Features Ease of Operation Data Storage Customized Calculations Support & Service

    7. BUCK/Cecil Instruments The COMPACT, “Stable-Beam” construction of the Single-Beam series uses the high-quality Cecil base instrument and high-performance components from BUCK Scientific

    8. BUCK/Cecil Instruments Best optical design and highest quality components resulting in superior specifications: Stray Light Noise Stability Baseline Flatness Accuracy (wavelength & photometric / ABS) Reproducibility (wavelength & photometric / ABS)

    9. BUCK/Cecil Series Series 2000 Single Beam, Scanning, 4nm bandpass Optional built-in printer & P-C Interface Many software options Most POPULAR Industrial system Applications: Bio-Quest, Aqua-Quest, Food-Quest series with Prep-Programmed Operations

    10. BUCK/Cecil Series Series 3000 Single Beam, Scanning, 1.8nm bandpass Optional built-in printer & P-C interface Many software features (Kinetics, Bio-Assay) Reflectance option: 3055 w/ Integrating Sphere

    11. BUCK/Cecil Series Series 7000/7000+/9000 Double-Beam, Scanning, some Models with variable 0.5 / 1.0 / 1.8-2.0 / 4.0nm bandpass Standard Printer & P-C interface, Data-Stream Many software features (Kinetics, Bio-Assay) Reflectance / Fluorescence / Turbidity options

    12. Sample Handling Options Cuvette - standard cell holder Quartz for UV range Glass & Plastic for Vis range 1-100mm Pathlengths Flow Cell - standard or micro flow (50?L) Uses Sipette Pump Optional Automatic Cell Changer

    13. Sample Handling Options Multi-Cell Changer 4, 6, or 8 Positions Manual, Automatic, Programmable Thermostatted Cell Holder Water-jacketted or Electronic Peltier system Batch Sampler - 40 Positions Carousel Specular & Integrating Sphere / Diffuse Reflectance

    14. Sample Handling Options

    15. Software Features

    16. Software Features

    17. APPLICATIONS Primarily used for QUANTITATIVE Analysis of Known Compounds, UV-Vis is one of the most popular techniques in the Pharmaceutical, Foods and Paints Industries, as well as Water Laboratories

    18. APPNOTE - Inorganic SAMPLE: River Water for PHOSPHATE PREPARATION: Digest w/ 10% HCl, add NH4MoO4 & Hydrazine reagent, boil till Blue color develops; measure at 810nm. ANALYSIS: CE-2041 or VIS-100 w/ USEPA 2-pt Calibration

    19. APPNOTE - Inorganic DATE Total-PO4 ortho-PO4 3-10-00 69 PPM 51 PPM 3-24-00 77 PPM 50 PPM 4-08-00 94 PPM 83 PPM 4-22-00 118 PPM 72 PPM 5-10-00 85 PPM 78 PPM ANION assays by Colorimetry are the ACCEPTED Standard World-Wide & much better (and less expensive) than Ion-Chromatography

    20. APPNOTE - Cosmetic SAMPLE: Color-Dyes for Hair Shampoos PREPARATION: Make 1:5 and 1:200 dilution of Dyes to compare to Samples ANALYSIS: CE-2041 or -3041 Scanning Spectrophotometer, 1cm. Glass Cuvette

    21. APPNOTE - Nutritional SAMPLE: Cereals and Grains (preserved) PREPARATION: Hydrolyze 2 grams w/ 5% HCl, extract w/ Hexane for 1:10 dilution ANALYSIS: CE-2041 or CE-3041 Scanning Spectrophotometer, scan range 200-450nm. For BHT, BHA and TBHQ Anti-Oxidants. Sample solutions run in 10mm. Quartz Cells

    22. APPNOTE - Nutritional Sample BHA BHT TBHQ [Wavelength 410nm. 360nm. 285nm.] Frosted Flakes 35 PPM 27 PPM 11 PPM Raisin Bran 16 PPM 41 PPM < 2 PPM Rice Krispies 23 PPM 11 PPM 4.5 PPM Scanning SPECTROPHOTOMETRY is an ideal method to evaluate YOUR OWN products, or material from the Competition

    23. Other SPECTRO Analyzers from BUCK BUCK Scientific also offers a series of inexpensive, high-performing and reliable Analytical Spectroscopy equipment for special Applications, Educational and Dedicated Testing procedures

    24. FLUORESCENCE SPECTROSCOPY (FLUOR) FLUORESCENCE is a form of Molecular “EMISSION” Spectroscopy; where a Sample in a special Cuvette is exposed to invisible, high-Energy UV radiation, and certain parts of the Organic Molecule will “give off” or EMIT lower-Energy VISIBLE Light radiation. Very specific & selective test for Drugs & Vitamins, Alkaloids & Toxins

    25. BUCK Filter Fluorometer The advantage of HIGH Sensitivity for most compatible materials makes Fluorescence a valuable analytical tool for Forensic & Drug applications

    26. BUCK Filter Fluorometer The availability of a wide assortment of Excitation and Emission Filters makes this portable FL-380 instrument very flexibility for a very low cost… ideal for Academic Teaching & Industrial QA/QC work

    27. Visible COLORIMETRY (COLOR) This is for use in Academic Teaching classes and for dedicated COLORIMETRIC Analyses for materials such as ANIONS (SO4, NO2, NO3, PO4, etc) and Visible Colors of compound such as Pigments, Chlorophyll and Dyes Covers the range from 320 - 950nm.

    28. BUCK Colorimeter The VIS-100 is a small, durable, self-calibrating unit that provides MEDIUM resolution @ 8nm. Bandpass, automatic 1-point Calibration & Data Outputs for a very low price

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