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Ivermectin Effects on Caenorhabditis elegans C.elegans

Background Information. . 1.Anti-parasitic MedicationStrongyloides stercoralis Onchocerciasis volvulus Pubic lice infestation Scabies Wuchereria bancrofti . 2 Mechanism of action Bind to GABA Channel Hyperpolarization by ?Cl Permeability. http://www.pnas.org/cgi/content/full/97/6/2674/F2.

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Ivermectin Effects on Caenorhabditis elegans C.elegans

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    1. Ivermectin Effects on Caenorhabditis elegans (C.elegans)

    2. Background Information

    3. Background Information Phylum Nematoda Feed on OP50 bacteria Pseudocoelomates Thrashing motion (contraction) Female deposit 100,000 or more egg/day Most individuals are Hermaphrodites Life Cycle : >12hours

    4. Research Purpose :To determine the effect of Ivermectin on wild type (C.elegans) Plan to extend strain Complications Method of introduction Drug Test Resistance strain

    5. Ivermectin response to OP50 bacteria Ivermectin has no effect on OP-50 bacteria

    6. Drug Introduction

    7. Wild type C.elegans

    8. Ivermectin: Wildtype worm

    9. Comparison

    10. Touch insensitive=Tapping

    11. Future Analysis Growth Assay: Count the number of gravid adult Reflects Pharyngeal function Mechanosensation: Touch sensitivity Neurological Response Motility assay: Number of worm still thrashing Locomotion

    12. References American Society of, Health-System Pharmacists Inc. Patient Drug Information From MedMaster. Maryland: 2007. Jamison, Dean T., and Joel G. Breman. Disease Control Priorities in Developing Countries. 2nd. New York: Oxford University Press, 1993. John Keane and Leon Avery Mechanosensory inputs influence Caenorhabditis elegans pharyngeal activity via ivermectin sensitivity genes. Genetics. 2003 May; 164(1): 153–162. PMCID: PMC1462566 Griffiths, Anthony J.F., and Jeffery H. Miller. HumanGenetics. 7th ed. New York: W.H. Freeman,1996. Hart, Anne C. "Behavior." Worm Book July 3 2006. 04 April 2008 <http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=wormbook. capter.7133>.

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