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Gene Expression in Early Stages of Micorrhizal Interactions

Gene Expression in Early Stages of Micorrhizal Interactions. By Sahel Arora. a mutualistic symbiosis between plant and fungus 2 major types Endomycorrhizae-- fungal component are Glomales Endomycorrhizae -- hyphae penetrates the outer cells of plant root. What is Mycorrhizae - review.

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Gene Expression in Early Stages of Micorrhizal Interactions

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  1. Gene Expression in Early Stages of Micorrhizal Interactions By Sahel Arora

  2. a mutualistic symbiosis between plant and fungus 2 major types Endomycorrhizae-- fungal component are Glomales Endomycorrhizae --hyphae penetrates the outer cells of plant root What is Mycorrhizae - review

  3. Review -- Ectomycorrhizae • associated plant species include majority of vascular plants • Essential for health and survival of conifers and hardwood species • the function depends on the ability of the fungal symbionts to take up nutrients

  4. Ectomycorrhizal Organ • Formation has 4 stages • Time of the process varies • Laccaria bicolor – Pinus resinosa interaction takes eight weeks • Pisolithus tinctorius – Eucalyptus globus takes about two weeks

  5. Fungal gene expression in early symbiotic interactions between Laccaria bicolor and red pine • Authors – G.K. Podila, J. Zheng, S. Balasubramaniam, S. Sundaram, S. Hiremath, J.H. Brand, and M.K. Hymes • Aim – to obtain a broad overview on various genes involved in the pre-infection stages of ectomycorrhizal formation, in the fungal partner • Uses L. bicolor for the fungal component and Pinus resinosa for the plant • it is known that signaling genes are involved in the early stage, the types of genes are unknown

  6. Materials and Methods • L. Bicolor was obtained, grown and maintained on MMN medium • In vitro system was used to isolate and analyze genes • cDNA library was constructed • cDNA – complimentary DNA; synthesized by using reverse transcriptase and ohage vector • cDNAs represent mRNAs during: a) its interaction with red pines for 6 – 72 hrs; b) free-living conditions

  7. Materials and Methods • suppression subtractive hybridization was used to generate cDNAs, enriched in IS sequences • These sequences were used as probes to screen the actions of cDNA • The resulting clones were converted to plasmid clones and purified • The clones went through the membrane array analyses and northern analyses for validation

  8. RESULTS AND DISCUSSION • The analyses indicated a relative increase in the expression of each gene in the interaction over the free-living state • Also, showed an increase in levels of clones tested, however there was a variation • The data identified several regulatory genes and insights into gene activities involved

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