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Fluorescence of GFP chromophore by cyclization reaction including rearrangement and oxidation

Green fluorescent protein ( GFP ). composed of 238 amino acids (26.9 kDa ), originally isolated from the jellyfish Aequorea victoria fluoresces green when exposed to blue light Used as a reporter of expression & biosensor The GFP gene can be introduced into

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Fluorescence of GFP chromophore by cyclization reaction including rearrangement and oxidation

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  1. Green fluorescent protein (GFP) • composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfishAequorea victoria • fluoresces green when exposed to blue light • Used as a reporter of expression & biosensor • The GFP gene can be introduced into • organisms (bacteria, yeast and other fungal cells, plant, fly, and mammalian cells) • 2008 Nobel Prize in Chemistry : Martin Chalfie, Osamu Shimomura and Roger Y. Tsien • A typical beta barrel structure Fluorescence of GFP chromophore by cyclization reaction including rearrangement and oxidation • Cleavage after protein synthesis • - digestive enzymes (pancreas, intestine) • - blood clotting factor (fibrinogen  firbrin) • - hormone, viral proteins

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