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Compact SPR Biosensor

Compact SPR Biosensor. CCD camera or photo diode array. Laser or LED. cover plate. display. d isposa ble sensor chip. prism. lens. polarizer. sample holder. digital window. sample cassette.

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Compact SPR Biosensor

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  1. Compact SPR Biosensor CCD camera or photo diode array Laser or LED cover plate display disposablesensor chip prism lens polarizer sample holder digital window sample cassette

  2. For the sample with low concentration, signal shift might be smaller than environmental drift change. Self-referencing eliminates influence by environment (excellent robustness against temperature and buffer change). PDMS flow cell analyte substrate z y x Self-referencing SPR Top view

  3. Au Au glass slide glass slide y x Experimental procedure Step 1 Top view PDMS micro flow cell solution Side view substrate width: 50 –300 μm for channels and spaces between channels Step 3 Step 2 channel width: 500 μm – 1 cm PDMS flow cell z substrate

  4. sensing area referencing area Au Au Au Au glass slide glass slide glass slide glass slide Multi-step functionalization procedure using mouse IgG as reference mouse IgG MUA antibody antigen Taya, figs for pat appl, Aug 2005, fr Fengyu REV Aug 30.ppt

  5. pbs mouse IgG 100 g/ml Mouse IgG absorption onto the gold surface (a) (c) (b) (d) y axis of sensor chip image pixel number

  6. Anti-CEA antibodies immobilized onto sensor chip (a) (c) (b) (d) y axis of sensor chip image pixel number

  7. Sensorgrams of TKH2 antibodies immobilizing onto sensor chip and STn antigens binding to TKH2 antibodies (b) (a) sensing (1) sensing (1) referencing (2) referencing (2) (1) - (2) (1) - (2) TKH2 20 g/ml STn 100 ng/ml

  8. sensing (1) referencing (2) (1) - (2) Sensorgrams of anti-CEA antibodies immobilizing onto sensor chip and CEA antigens binding to antibodies (b) (a) sensing (1) referencing (2) (1) - (2) anti-CEA antibody 20 g/ml CEA 10 ng/ml

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