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Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates

Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates. Ji Eun Lee MD PhD, Jong Soo Lee MD PhD Department of Ophthalmology, Graduate School of Medicine, Pusan National University. The authors have no financial interest in the subject matter of this e-poster.

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Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates

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  1. Evaluation of Cysticidal Effects of Contact Lens Disinfectant onAcanthamoeba Isolates Ji Eun Lee MD PhD, Jong Soo Lee MD PhD Department of Ophthalmology, Graduate School of Medicine, Pusan National University The authors have no financial interest in the subject matter of this e-poster.

  2. Purpose • To evaluate the cysticidal effect of • 5 kinds of commercially available contact lens disinfectants • against 2 clinical isolates of Acanthamoeba.

  3. Methods • Five kinds of commercially available contact lens disinfectants were soaked with cysts of Acanthamoeba ludgdunesis and castellaniiat the concentration of 103, 104, and 105 cells/ml for 1 and 4 or 6 hours. • Cysts which were not excysted in 7 days after treatment were recognized to be killed. • Morphologic changes were evaluated by electron microscopic observation.

  4. Table 1. Formula of contact lens disinfectants Methods

  5. Results • Contact lens disinfectants which contain myristamidopropyl dimethylamine (MAPD) showed the best cysticidal effect. These disinfectants demonstrated a cysticidal effect on both Acanthamoeba species of all concentrations in 6-hour treatment. • Contact lens disinfectants which contain polyhexamethylene biguanide (PHMB) did not demonstrate cysticidal effect, except for Acanthamoeba castellanii at the concentration of 103 cells/ml, in either 4- or 6-hour treatment. Table 2. Viability of Acanthamoeba cyst

  6. Results • Separation of plasma membrane from endocyst and damage of organelles were prominent in cases showing a cysticidal effect. Figure 1. Transmission electron micrograph of Acanthamoeba castellani cysts after exposure to (A) LOEYS HAPPY TEARS for 1 hour and (B) OPTI-FREE express for 6 hours (bar length 2 um, original magnification, A & B: X 3,000∼4,000). Separation of plasma membrane from endocyst and invagination of plasma membrane was observed (arrows). The cytoplasm and organelles were deformed and aggregated in severely damaged cyst and the leakage of intranuclear materials was prominent.

  7. Conclusions • Contact lens disinfectant which contains MAPD may be helpful in preventing the Acanthamoeba keratitis. • A higher concentration of PHMB is required to be effective in preventing Acanthamoeba keratitis.

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