80 likes | 356 Views
Matrix Metalloproteins (MMPs) . How Zn2+ is involved in the catalytic domain and how it can be inhibited. What are MMP’s?. Pro-domain catalytic domain . Similarities/differences between catalytic & pro domains of MMPs. How are MMP’s involved in disease?. Mechanism.
E N D
Matrix Metalloproteins (MMPs) How Zn2+ is involved in the catalytic domain and how it can be inhibited.
What are MMP’s? • Pro-domain • catalytic domain Similarities/differences between catalytic & pro domains of MMPs
How was this structure discovered? • XANES (X-Ray Absorption Near-edge Structure) • Zn2+ coordination • Different pH • Mutating active site residues These people don’t consider the enzyme in its inactive state, ie. cysteine switch.
How is the active site inhibited? • Understanding the catalytic domain leads to an understanding of how it is inhibited. • And here’s how… • How the Zn coordination is altered ***Focus on techniques used small molecule screening for development of drugs TIMPs
Future Endeavours • Why does deletion of different MMPs cause a variety of phenotypic mutations?