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Normal recombinant PrP. Thioflavin T ( ThT ). Test sample. +. +. sCJD brain 1:4,000,000. sCJD nasal brushing. 96-well plate. Non-CJD nasal brushing. Shaking fluorescence plate reader. +. prion seed. PrP amyloid with fluorescent ThT stain. Normal recombinant PrP + ThT.
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Normal recombinant PrP ThioflavinT (ThT) Test sample + + sCJD brain 1:4,000,000 sCJD nasal brushing 96-well plate Non-CJD nasal brushing Shaking fluorescence plate reader + prion seed PrP amyloid with fluorescent ThT stain Normal recombinant PrP + ThT
sCJD brain 1:4,000,000 sCJD nasal brushing Non-CJD nasal brushing