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Opaque 96-well

Versant Branched-DNA assay. Label probes + Alkaline phophatase. Pre-amplifier probe. Amplifier probes. Target probe. HIV RNA (from lysed particles). Capture probe. Opaque 96-well . # of RNA strands is proportional to chemiluminescence intensity from

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Opaque 96-well

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  1. Versant Branched-DNA assay Label probes + Alkaline phophatase Pre-amplifier probe Amplifier probes Target probe HIV RNA (from lysed particles) Capture probe Opaque 96-well • # of RNA strands is proportional to chemiluminescence intensity from • alkaline phosphatase reaction

  2. Wave80’s Versant Branched-DNA assay A ~ 734,000 cp/mL B ~ 45,000 cp/mL C ~ 4,500 copies/mL E ~ 90 copies/mL D ~ 450 copies/mL Neg. control • Reliable detection threshold ~ 450 copies HIV RNA/mL

  3. Our b-DNA assay has lower sensitivity than Wave80 A Hi B C C ~ 4,500 copies/mL Lo D E Neg. ctrl F • Plate reader either has lower sensitivity / dynamic range, or we could have • overall RNA breakdown

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