1 / 10

SCHOTT NEXTERION MTP-96

SCHOTT NEXTERION MTP-96. Maximum & Minimum filling volumes per well. Experimental Details. Substrate tested: - MTP 2-2259 without patterning 18.01.11 Buffer Solutions tested: - PBS (1x) with 0.08 mg/ml Dye 5 - PBST 0.1% Tween with 0.08 mg/ml Dye 5. Filling Scheme.

chrissanchez
Download Presentation

SCHOTT NEXTERION MTP-96

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SCHOTT NEXTERION MTP-96 Maximum & Minimum filling volumes per well

  2. Experimental Details • Substrate tested: - MTP 2-2259 without patterning 18.01.11 • Buffer Solutions tested: - PBS (1x) with 0.08 mg/ml Dye 5 - PBST 0.1% Tween with 0.08 mg/ml Dye 5 Corinna Schwung, 06.01.2020

  3. Filling Scheme These wells were filled up to maximum liquid level. Corinna Schwung, 06.01.2020

  4. 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H Results from adding 10 – 120 µl per well • Solution pipetted into upper left corner • MTP was gently shaken PBST • Minimum Volumes • PBST: 40 µl • PBS: 50 µl PBS 10 µl 120 µl Corinna Schwung, 06.01.2020

  5. 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H Results from adding 10 – 240 µl per well 130 µl 240 µl PBST PBST 10 µl 120 µl PBS PBS 130 µl 240 µl Corinna Schwung, 06.01.2020

  6. Results from adding 10 – 240 µl per well • Safe filling volume is about 160 µl for PBS, 130 µl for PBST PBST PBS 240 µl 130 µl 140 µl 150 µl 160 µl 170 µl 180 µl 190 µl 200 µl 210 µl 220 µl 230 µl PBS PBST 240 µl 230 µl 220 µl 210 µl 200 µl 190 µl 180 µl 170 µl 160 µl 150 µl 140 µl 130 µl Corinna Schwung, 06.01.2020

  7. Results from adding 10 – 240 µl per well PBS 190 µl 200 µl 210 µl 220 µl 230 µl 240 µl • The maximum filling level was not reached with these volumes PBST 240 µl 230 µl 220 µl 210 µl 200 µl 190 µl Corinna Schwung, 06.01.2020

  8. Results from adding 320 – 400 µl per well PBS • Maximum Volume per well • PBS: 410 µl • PBST: 340 µl 370 µl 380 µl 390 µl 400 µl PBST 350 µl 340 µl 330 µl 320 µl Corinna Schwung, 06.01.2020

  9. Conclusions • The minimum volume that will cover the well bottom is: • 40 microlitres (with detergent) • 50 microlitres (without detergent) • The maximum recommended „safe“ working volume is: • 130 microlitres (with detergent) • 160 microlitres (without detergent) Corinna Schwung, 06.01.2020

  10. SCHOTT NEXTERION MTP-96 Maximum & Minimum filling volumes per well

More Related