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The Effect of Nicotine on Lumbriculus Variegatus’s Pulse Rate per Minute: GCC Bio 100 Lab Poster

The Effect of Nicotine on Lumbriculus Variegatus’s Pulse Rate per Minute: GCC Bio 100 Lab Poster. By: Joe Madejski , Aaron Chen.

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The Effect of Nicotine on Lumbriculus Variegatus’s Pulse Rate per Minute: GCC Bio 100 Lab Poster

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  1. The Effect of Nicotine on Lumbriculus Variegatus’s Pulse Rate per Minute: GCC Bio 100 Lab Poster By: Joe Madejski, Aaron Chen Procedure : The worms were removed from the refridgeration10+ minutes before hand. We started to test the how long it would take for the worms to perish using the .1mM concentration, which was our strongest. The worms could survive for approximately 2 minutes, so we decided to expose the worms for1.5 minutes in the stock solution. Our first test was using the .1mM solution. One at a time, we exposed the worms to 5 milliliters of the stock solution (enough to immerse the worm) for 1.5 minutes, and then counted their pulse under a microscope on low power (4x). Once we had recorded the pulse rate, we moved the worms into a recovery petri dish so that they would not die or affect the other worms with the their nicotine secretions. This was repeated for ten trials. We did the same things for the .05mM and the .01mM concentrations, as well as our control (spring water). Introduction:Nicotineis an acetylcholine aggravator (a stimulant) that is skin soluble and is found in the nightshade family of plants. It has a chemical composition of C10H14N2. Nicotine was and is a very common and popular pesticide and is used by plants as an anti-herbivore chemical. In order to test the effects of it on pulse rates, using Lumbriculus Variegatus (California blackworm) is a good choice considering it has a very thin epidermal layer and is very small so the nicotine would transfer into the worm and effect it within a short period of time. The worm also has a translucent skin, making it easy to observe the changes in pulse rate per minute. Results – After testing, we found that all solutions increased the pulse rate by a significant amount. The spring water test resulted in a ppm (pulse per minute) mean of 13. For the .1mM solution, the ppm mean increased by 2; for the .05mM solution, it increased by 1.3 ppm; for the .01mM solution, it increased by 3.4 ppm. Conclusion: As you can see in the graph and it’s data table to the left, our results supported our hypothesis. In our testing of the .1mM solution, one worm did end up perishing. The worm was smaller than the rest, so it is possible that the nicotine affected it sooner and much stronger than the rest of the tested worms. The control was also much lower than previous experimentation suggested, but this did eventually even out since the stimulant results were roughly proportional to the previous experiment’s results. Another anomaly is that the lowest concentration of the nicotine concentration had the highest average increase in pulse rate. The other solutions could have been affecting the worm to strongly, so their ppm rate would decrease by the time we tested them. Materials : -Microscope -Hot Plate -Flasks -Slide with valve -cover slip -Beaker -Goggles -Petri dishes -Spring Water -Graduated Cylinder -Pipettes -Gloves –filter –funnel –sink -.1, .05 and .01 mille Molar Concentration of Nicotine solution -paper towels IndependentVariable: Nicotine solution concentration DependentVariable: Pulse rate per minute Control: Spring water Constants: Type of spring water, time exposure to Nicotine, storage area, temperature of the room, temperature of stock solution, type of microscope. Data Table Trial Control .1mM .05mM .01mM 1 10 15 10 17 2 12 12 14 16 3 12 0 14 15 4 13 16 11 16 5 14 19 16 17 6 14 18 16 16 7 13 16 16 16 8 15 17 14 17 9 13 16 12 14 10 14 21 20 20 Hypothesis - If worms are treated with nicotine in solution concentrations of .1, .05, and .01mM concentrations, then their pulse rate per minute should be higher compare to the ones treated in spring water. -Bohrer, K. E. (n.d.). Effects of Drugs on Pulsation Rate of Lumbriculus. Retrieved September 15, 2013, from http://www.ableweb.org/volumes/vol-27/07_Bohrer.pdf -Campain, J. A. (2004, February 24). Nicotine: Potentially a Multifunctional Carcinogen? Retrieved September 13, 2013, from TOXICOLOGICAL HIGHLIGHT: http://toxsci.oxfordjournals.org/content/79/1/1.full.pdf -NIOSH Publications and Products. (n.d.). Retrieved September 13, 2013, from Center for Disease Control and Prevention: http://www.cdc.gov/niosh/idlh/54115.html References:

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