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PROTEINS

PTT 202: ORGANIC CHEMISTRY FOR BIOTECHNOLOGY. PROTEINS. PREPARED BY: NOR HELYA IMAN KAMALUDIN helya@unimap.edu.my. Introduction of Proteins. Separation of Proteins. Precipitation. Electrophoresis. Technical aspects of electrophoresis. 1. Supporting medium.

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PROTEINS

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  1. PTT 202: ORGANIC CHEMISTRY FOR BIOTECHNOLOGY PROTEINS PREPARED BY: NOR HELYA IMAN KAMALUDIN helya@unimap.edu.my

  2. Introduction of Proteins

  3. Separation of Proteins

  4. Precipitation

  5. Electrophoresis

  6. Technical aspects of electrophoresis

  7. 1. Supporting medium

  8. 2. Iso-electric focusing technique

  9. 3. Buffer pH

  10. 4. Buffer concentration

  11. 5. The methods of capillary electophoresis performed

  12. Quantitative aspects for electrophoresis

  13. 1. Semi-quantitative method

  14. 2. Alternative quantitative method

  15. Immunological methods

  16. The steps of immunoblotting

  17. The steps of immunoblotting (cont)

  18. Immunoaffinity purification

  19. Chromatographic methods

  20. THANK YOU..

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