1 / 10

pKa PRO ™ System: Competing Technologies

pKa PRO ™ System: Competing Technologies. Competing Technologies for pKa Analysis. Potentiometry UV Spectrophotometry Single column CE Single column CE-MS. CONFIDENTIAL. Potentiometry. Low sample throughput 30 samples/24 h for aqueous pKa Requires mg of sample

clark-dickerson
Download Presentation

pKa PRO ™ System: Competing Technologies

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. pKa PRO™ System: Competing Technologies

  2. Competing Technologies for pKa Analysis • Potentiometry • UV Spectrophotometry • Single column CE • Single column CE-MS CONFIDENTIAL

  3. Potentiometry • Low sample throughput • 30 samples/24 h for aqueous pKa • Requires mg of sample • Impurities can interfere w/measurement • Can measure non-UV absorbing samples • Extensively developed protocols • Complicated, time consuming data analysis • Can use multiple co-solvent systems • Methanol, DMSO, ACN, Ethanol, DMF Sirius GLpKa • pKa PRO™system provides: • Increased sample throughput • Much lower sample consumption • Separation/isolation of impurities • More intuitive data analysis • Indication of overall compound charge • Versatile platform (log P, chiral, etc) CONFIDENTIAL

  4. Comparisons Between pKa PRO™ and Sirius GLpKa CONFIDENTIAL

  5. Potentiometry w/UV-Spectrophotometry • Low sample throughput • 30 samples/24 h for aqueous pKa • Requires 150 ug of sample • Impurities can interfere w/measurement • Must absorb UV, sensitive to compound structure • Ionization center must be within 2-3 bonds of chromophore • Complicated, time consuming data analysis Sirius GLpKa w/DPAS • pKa PRO™system provides: • Increased sample throughput • Measurement NOT sensitive to compound structure • Lower sample consumption • Separation/isolation of impurities • More intuitive data analysis • Indication of overall compound charge • Versatile platform (log P, chiral, etc) CONFIDENTIAL

  6. Spectral Gradient Analysis UV-Spectrophotometry • High sample throughput • Claim 300 samples/24 h for aqueous pKa • Requires ~100 ug of sample • Impurities can interfere w/measurement • Must absorb UV, sensitive to compound structure • Ionization center must be within 2-3 bonds of chromophore • Some pKa values “invisible” to instrument • Complex data analysis, not easily understood • Co-solvent methods not fully developed Sirius SGA • pKa PRO™system provides: • Similar throughput • Measurement NOT sensitive to compound structureSeparation/isolation of impurities • More intuitive data analysis • Indication of overall compound charge • Versatile platform (log P, chiral, etc) CONFIDENTIAL

  7. Comparisons Between pKa PRO™ and Sirius ProfilerSGA Information From: (1) Box, K.; Bevan, C.; Comer, J.; Hill, A.; Allen, R.; Reynolds, D. Anal. Chem.2003, 75, 883-892. CONFIDENTIAL

  8. Information From: (1) Box, K.; Bevan, C.; Comer, J.; Hill, A.; Allen, R.; Reynolds, D. Anal. Chem.2003, 75, 883-892. CONFIDENTIAL

  9. Single Column Capillary Electrophoresis • Same principles, similar results to the pKa PRO™ • Slightly better detection sensitivity • Low throughput (10 samples/day for aqueous pKa) • No application specific software available • Co-solvent measurements impractical • Low throughput • No software available for calculations • No consumables commercially available • Estimated system cost: ~$60,000 US Beckman P/ACE™ MDQ DNA System Also: Agilent single channel CE • pKa PRO™system provides: • Greatly increased sample throughput • Dedicated software for pKa analysis • Co-solvent measurement capabilities • Full line of ready-to-use reagents • Predefined methods for greater ease-of-use CONFIDENTIAL

  10. Single Column CE-MS • Same principles, similar results to the pKa PRO™ • ~10X better detection sensitivity • Can measure pKa for non-UV absorbing samples • Low throughput (pool samples to increase throughput) • No application specific software available • Need to deconvolute pooled samples by MW! • No pKa software commercially available • Co-solvent measurements impractical • Low throughput • No software available for calculations • No consumables commercially available • High cost of ownership; requires highly technical user • Estimated system cost: >$100,000 US Agilent CE-MS System • pKa PRO™system provides: • Greatly increased sample throughput • Dedicated software for pKa analysis • Co-solvent measurement capabilities • Lower cost of ownership; less technical resources required • Full line of ready-to-use reagents • Predefined methods for greater ease-of-use CONFIDENTIAL

More Related