Vibrio vulnificus Damages Macrophages during the Early Phase of Infection

1. Vibrio vulnificus Damages Macrophages during the Early Phase of Infection Takahiro Tsuchiya, Eriko Mitsuo, Nahoko Hayashi, Yuuko Hikita, Hiroshi Nakao, Shigeo Yamamoto, Katsushiro Miyamoto, and Hiroshi Tsujibo Infection and Immunity, September 2007, Vol. 75, No. 9 Class:Infection disease lecture Speaker: Lin Chien Chou

2. Experimental • Colony morphology of Vibrio vulnificus. • Survival of V. vulnificus-inoculated mice. • Distribution of V. vulnificus in mice. • Exudation of leukocytes to the peritoneal cavity. • Cytotoxic activity of V. vulnificus in various cells. • Effect of leukocytes on bacterial growth • Effect of leukocytes on the survival of V. vulnificus-inoculated mice.

3. Strain M2799 strain JCM3731 FIG. 1. Morphology of V. vulnificus colonies.

4. FIG. 2. Survival of V. vulnificus-inoculated mice.

5. 1hour □M2799 ■ JCM3731 100X 3hours FIG. 3. Detection of V. vulnificus in various tissues.

6. □M2799 ■ JCM3731 sham inoculated PECs isothiocyanate-conjugated anti-Gr1 /R-phycoerythrin-conjugated anti-Mac1. FIG. 4. Analysis of exudate leukocytes in the peritoneal cavity.(In vivo)

7. control

8. FIG. 5. Effect of leukocytes on bacterial growth.(In vitro) □Macrophage ■ Neutorphils Without Mac& Neu 16X

9. V.V cytotoic Mac wherease cloud protect host survival FIG. 6. Effect of leukocytes on survival of strain M2799-inoculated mice(in vivo) ◆with Macrophge cell ● with Neutrophils cell ▲ with P-buffer

10. Discussion-1

11. Discussion-2 Vibrio vulnificus Caspase 1 Apoptosis Neutrophile Cytokine Macrophage The issue of Virulence factor attack to Macrophage