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CULTIVAREA SI IDENTIFICAREA VIRUSURILOR

CULTIVAREA SI IDENTIFICAREA VIRUSURILOR. Structura foarte simpla agenti infectiosi acelulari contin acizi nucleici si invelis proteic protector sunt adaptati la un parazitism obligator intracellular se pot cultiva doar in celula vie. VIRUSURILE. PARAZITISM ABSOLUT AL CELULELOR VII

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CULTIVAREA SI IDENTIFICAREA VIRUSURILOR

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  1. CULTIVAREA SI IDENTIFICAREA VIRUSURILOR

  2. Structura foarte simpla • agenti infectiosi acelulari • contin acizi nucleici si invelis proteic protector • sunt adaptati la un parazitism obligator intracellular • se pot cultiva doar in celula vie

  3. VIRUSURILE • PARAZITISM ABSOLUT AL CELULELOR VII • SISTEME CELULARE - ANIMALE DE EXPERIENTA - OUA EMBRIONATE - CULTURI DE CELULE

  4. VIRUSURILE • EVIDENTIEREA VIRUSURILOR - EXAMINAREA MICROSCOPICA A PRODUSELOR PATOLOGICE SI A TESUTURILOR INFECTATE (MODIFICARI SPECIFICE VIRUSURILOR) - CULTIVAREA SI IDENTIFICAREA PRIN METODE SPECIFICE • DEMONSTRAREA UNEI CRESTERI A TITRULUI ANTICORPILOR SPECIFICI (FAZA ACUTA/ CONVALESCENTA)

  5. CULTIVAREA VIRUSURILORINOCULAREA LA ANIMALELE DE EXPERIENTA • SUNT UTILIZATE ANIMALE MICI • EXISTA DIFERENTE DE SENSIBILITATE - SPECIE - RASA - VARSTA

  6. CULTIVAREA VIRUSURILORCAILE DE INOCULARE • SUBCUTANATA • INTRAVENOASA • INTRANAZALA • INTRAPERITONEALA • SCARIFICARE CUTANATA SAU CORNEEANA • INOCULARE INTRACEREBRALA

  7. CULTIVAREA VIRUSURILORCAILE DE INOCULARE • REPRODUC MODALITATEA DE PATRUNDERE A VIRUSULUI IN ORGANISMUL UMAN • ARTIFICIALE

  8. CULTIVAREA VIRUSURILORDEMONSTRAREA REPLICARII VIRALE • LEZIUNI MACROSCOPICE/ MICROSCOPICE • REPRODUCTIBILITATEA LEZIUNILOR

  9. VIRUSURILE COXSACKIE • INOCULARE SUBCUTANATA LA SOARECELE SUGAR - COXSACKIE A - PARALIZIE FLASCA - MIOZITA GENERALIZATA - COXSACKIE B – PARALIZIE SPASTICA - MIOZITA LOCALIZATA - LEZIUNI SNC SI VISCERE

  10. VIRUSURILE COXSAKIE VEZICULE BOALA MANA-PICIOR-GURA

  11. VIRUSURILE GRIPALE • INSTILARE INTRANAZALA LA SOARECELE ADULT • PNEUMONIE INTERSTITIALA CARACTERISTICA

  12. GRIPA…AVIARA ARCA LUI NOE SI GRIPA AVIARA

  13. VIRUSURILE HERPETICE • SCARIFICARE CORNEANA LA IEPURE • APARITIA KERATOCONJUNCTIVITEI KERATOCONJUNCTIVITA LA IEPURE DUPA SCARIFICARE CORNEEANA

  14. VIRUSURILE HERPETICE HERPES LABIAL HERPES GENITAL

  15. VIRUSURILE HERPETICE NERV MAXILAR NERV OFTALMIC

  16. VIRUSURILE HERPETICE NERVI INTERCOSTALI

  17. VIRUSURILE HERPETICEHERPES ZOSTER VEZICULE LA NIVEL OROFARINGIAN VEZICULE SI CRUSTE PE TRAIECTUL N. OFTALMIC / MAXILAR

  18. VIRUSURILE HERPETICEHERPES ZOSTER HERPES ZOSTER INTERCOSTAL

  19. VIRUSUL RABIC • INOCULARE INTRACEREBRALA LA SOARECE • ENCEFALITA RABICA • HEMATOXILINA-EOSINA CORPUSCULI BABES-NEGRI

  20. VIRUSUL RABIC CORPUSCULI INTRACITOPLASMATICI- BABES ERNST

  21. CULTIVAREA VIRUSURILOROUL DE GAINA EMBRIONAT

  22. CULTIVAREA VIRUSURILORINOCULAREA IN CULTURI DE CELULE RECIPIENTE PENTRU CULTURI DE CELULE VERIFICAREA PH-ULUI MEDIULUI DE CULTURA

  23. CULTIVAREA VIRUSURILORINOCULAREA IN CULTURI DE CELULE CONTAMINAREA CULTURILOR DE CELULE

  24. CULTIVAREA VIRUSURILORINOCULAREA IN CULTURI DE CELULE INCUBATOR PENTRU CULTURILE DE CELULE

  25. MRC-5 Human lung Fibroblast HELA  Human cervix Epithelial VERO  African Green Monkey Kidney Epithelial NIH 3T3Mouse embryo Fibroblast L929Mouse connective tissue Fibroblast CHO  Chinese Hamster Ovary Fibroblast BHK-21Syrian Hamster Kidney Fibroblast HEK 293Human Kidney Epithelial HEPG2Human Liver Epithelial EXEMPLE DE LINII CELULARE STABILIZATE

  26. EXEMPLE DE LINII CELULARE STABILIZATE BAE-1  Bovine aorta Endothelial NSO  Mouse myeloma Lymphoblastoid-like U937Human Hystiocytic LymphomaLymphoblastoid Namalwa Human Lymphoma Lymphoblastoid HL60  Human Leukaemia Lymphoblastoid-like WEHI Mouse B-cell Lymphoma Lymphoblastoid YAC 1 Mouse Lymphoma Lymphoblastoid U 266B1 Human Myeloma Lymphoblastoid SH-SY5Y  Human neuroblastoma Neuroblast

  27. CULTURI DE CELULE A. CULTURA PRIMARA B. CULTURA 16 ZILE C+D EVIDENTIERE PRIN IMUNOHISTOCHIMIE A+B PRIMA GENERATIE C+D A 2-A GENERATIE A TESUT OSOS SANATOS B NODUL CALCIFICAT C PASAJUL 2

  28. INCLUZII CELULARE INTRACITOPLASMATICE V. RABIC INCLUZII INTRACITOPLASMATICE

  29. INCLUZII INTRACITOPLASMATICE V.EBOLA INCLUZII INTRACITOPLASMATICE HEPATOCITE

  30. REACTII ANTIGEN-ANTICORP UTILIZATE IN VIRUSOLOGIE • EVIDENTIEREA ANTIGENELOR (VIRUSURILE) • EVIDENTIEREA ANTICORPILOR (CRESTEREA DE CEL PUTIN 4 X A TITRULUI)

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