Understanding Immune System: Organs, Cells, and Molecules

1. Chapter IIIGeneral introduction of immune system

2. Immune system immune organs or tissues bone marrow thymus spleen lymph nodes Immune cells : lymphocytes : T cells , B cells and NK cells phagocytes: Dendritic cells ,macrophage ,PMN immune molecules : antibody complement (补体） cytokines adhesive molecules

4. Immune organs • Central immune organs or primary lymphoid organs The site that lymphocytes generateand mature into functional cells Bone marrow and Thymus • Peripheral immune organs or secondary lymphoid organs and tissues The sites that T and B lymphocytes reside in and respond to antigens spleen , lymph nodes , mucosal or cutaneous lymphoid tissues

6. Section I Central immune organs

7. I. Bone marrow 1. The bone marrow is the site of generation of all circulating blood cells in the adult, including immature lymphocytes

10. 2. The bone marrow is the site of B cell maturation

11. 3. The sites that secondary humoral immunity carries through

14. II. Thymus 1. The structure of thymus Cortex outer cortex : immature T cells ( thymus cells) inter cortex : thymus epithelial cell, macrophage , DC Medulla Mature T cells + thymus epithelial cell, macrophage , DC

15. The structure of thymus

16. 2. The function of thymus • The thymus is the site of T cell maturation • Immune regulation:Secrete cytokine and thymulin-like molecules Thymosin（胸腺素）， thymulin-（胸腺刺激素） thymopoietin（胸腺生成素） To promote the proliferation , differentiation of thymocytes

18. Section II Peripheral Immune tissues and organs

19. The immune organs /tissues or secondary lymphoid organs/tissues are sites that T and B lymphocytes reside in and respond to antigens spleen lymphoid nodes mucosal immune system (mucosal or cutaneous-associated lymphoid tissues, MALT,)

20. I. Spleen 1. The Structure of spleen • white pulp Central arteriole peripheral lymphoid sheaths (PALS) T–cell area : around the central arteriole contain T cells , dendritic and macrophage B -cell area: lymphoid follicles contain B cells and macrophage and dendritic cells primary lymphoid follicle secondary lymphoid follicle • Red pulp: cellular cords : B cells , plasma cells and macrophage ,DC venous: blood

23. 2. Function of spleen • The site of T and B cells residence B cells : thymus-independent area T cells : Thymus –dependent area • Site of immune response to antigens • Synthesize some bioactive substance component of complement • Filtering blood

24. II. Lymphoid nodes

26. Structure of Lymphoid node Cortex : Outer cortex:thymus-independent area : B cell-rich area lymphoid follicle/lymphoid nodule : primary lymphoid follicle : without germinal center mature and naïve B cells secondary lymphoid follicle : with germinal center activated B cells and memory B cells and macrophage Paracortex: T cell-rich area+ dendritic cells post-capillary venules, PCV or high endothelial venules Medulla : medulla cords: macrophage and plasma cells medulla sinus

28. 2. Function of lymphoid node • The site of T and B cells residence B cells : thymus-independent area T cells : Thymus –dependent area • Site of immune response to antigens • Part of Recirculation of lymphocytes • Filtering blood

31. III. Skin and mucosal immune system • Skin-associated lymphoid tissue • Mucosal immune system, also named as mucosa-associated lymphoid tissue, MALT) is in the epithelia , lamina propria and submucosal area of gastrointestinal, respiratory and genitourinary tract

32. （I）The constitution of MALT • Gut-associated lymphoid tissue, GALT • Nasal-associated lymphoid tissue, NALT tonsils • Bronchial-associated lymphoid tissue, BALT : nodule

33. Gut-associated lymphoid tissue, GALT (1) mucosa Epithelia: intraepithelial lymphocytes (IEL) : T cells M cells lamina propria: lymphoid nodule aggregated lymphoid nodule or Peyer’s patches: B cells and T cells solitary lymphoid nodule: B cells and T cells (2) submucosa Scattered lymphoid tissues : T cells

34. intraepithelial lymphocytes (IEL) : T cells In mice : 40-50% of lymphocytes come from thymus ,  T cells 50-60% of lymphocytes come from non-thymus ( from bone marrow) ,  T cells

35. M cells : • Some of the epithelial cells overlying Peyer’s Patches are specialized membranous( M ) cells. • M cells lack microvilli,are actively pinocytic, and transport macromolecules from the intestinal lumen into subepithelial tissues. • They are thought to play an important role in delivering antigen to Peyer’s patches

36. submucosa

37. CD4+ 

38. normal graft contral SGVHD CD4/CD8  T receptor CD4/CD8  T receptor

39. （II）The function and features of MALT • First defense line • Site of immune response to antigens • Secrete sIgA

40. Section III Lymphocytes homing and recirculation I. Lymphocyte homing The process by which particular subsets of lymphocytes selectively enter some tissues but not others is called lymphocyte homing

41. II. lymphocyte recirculation The continuous movement of lymphocytes between blood stream and peripheral lymphoid tissues is called lymphocyte recirculation 1. Pathway of lymphocyte recirculation 2. Significance of lymphocyte recirculation

43. Lymphoid node-blood recirculation • Blood • Enter into paracortex of Lymphoid node by high endothelial venules (HEV) in • efferent lymphatics Thoracic duct venule

45. Spleen-Blood recirculation • Blood • Enter into white pulp of spleen via central artery • spleen venule

46. Tissue –lymphoid node-blood recirculation Tissues Afferent lymphatics Lymphoid node efferent lymphatics Blood

47. Significance of lymphocyte recirculation • Renew the lymphocytes in peripheral lymphoid organs • Maximize the chances of specific encounter with antigen and initiation of adaptive immunity • Enable lymphocytes to lymphoid tissue throughout body and facilitate immune response to antigen

48. Review 1.Terms MALT, Central immune organs, Peripheral immune organs, lymphocyte recirculation, Lymphocyte homing 2. Questions : Significance of lymphocyte recirculation The function of thymus, bone marrow, spleen , LN and MALT