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Friday, May 4, 2012

Friday, May 4, 2012. Homework: None. Entry 46: Dihybrid Punnett Squares -5/2/12 Attach dihybrid sheet Entry 47: pGLO intro questions - 5/4/12 Glue in the questions, read info sheet, answer questions. PLASMIDS. CHROMOSOME. Bacterial plasmids.

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Friday, May 4, 2012

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  1. Friday, May 4, 2012 Homework: None

  2. Entry 46: DihybridPunnettSquares-5/2/12 Attach dihybrid sheet Entry 47: pGLO intro questions- 5/4/12 Glue in the questions, read info sheet, answer questions

  3. PLASMIDS CHROMOSOME

  4. Bacterial plasmids Most bacterial DNA is in a single large chromosome, but some DNA is in small circles called plasmids. • Plasmids can have genes which make bacteria resistant to antibiotics • Plasmids can be passed between bacteria

  5. CUT OUT THE PAPER STRIP WITH THE UNEVEN ENDS-TAPE TOGETHER TO MAKE A RING LIKE THE PICTURE ON THE RIGHT GENE FOR ANTIBIOTIC RESISTANCE

  6. DNA from jellyfish with a gene for making GFP Plasmid with restriction enzyme sites and a gene for antibiotic resistance

  7. Gene Enhancer Promoter Coding sequence Terminator GFP GENE AMPICILLIN RESISTANCE GENE (bla) THE PLASMID

  8. Plasmids can be manipulated easily in the laboratory • Plasmids can be collected from bacteria • Restriction enzymes can cut specific sequences of DNA • Ligase (enzyme) can paste pieces of DNA together • Bacteria can be transformed by taking in plasmids given to them

  9. Cutting and Pasting DNA • Restriction enzymes act like molecular scissors – they cut the DNA! • Ligaseacts like molecular glue – it is used to reconnect the DNA that has been cut by restriction enzymes.

  10. GATCC G G CCTAG A Restriction Enzyme: BamH I

  11. Transforming Bacteria • When a bacterial cell takes in a plasmid from the environment, it has new DNA (and therefore, new traits). • Scientists say that the bacteria has been transformed.

  12. PLASMIDS WITH GFP GENE AND ANTIBIOTIC RESISTANCE GENE PORES PLASMIDS

  13. PLASMIDS WITH GFP GENE AND ANTIBIOTIC RESISTANCE GENE

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