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Optogenetic self stimulation of VTA dopamine neurons induces robust reward seeking

Optogenetic self stimulation of VTA dopamine neurons induces robust reward seeking. Mamelo Cami Other lasts papers. Marc Turiault. Systems X, July 11-13 th 2011. Swiss National Science Foundation. Amyg PPT. NAcc. A ddictive drugs activates the dopamine system.

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Optogenetic self stimulation of VTA dopamine neurons induces robust reward seeking

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  1. Optogeneticself stimulation of VTA dopamine neurons induces robust reward seeking Mamelo Cami Other lasts papers. Marc Turiault Systems X, July 11-13th 2011 Swiss National Science Foundation

  2. Amyg PPT NAcc Addictive drugs activates the dopamine system Luscher and Ungless 2006

  3. Cocaine in vivo induces LTP in Dopamine neurons Amyg PPT 24 h Ex vivo recordings On VTA slices Cocaine injection NAcc Ils le voient a quelpotentielce courant ? NMDA AMPA 50 ms AMPA/NMDA ratio Ungless et al. Nature, 2001 and Saal et al. Neuron, 2003

  4. In vivo cocaine induced AMPA rectification Amyg PPT NAcc Sal Cocaine Morphine Nicotine Brown et al. 2010

  5. The addictive properties of cocaine depend on DAT Amyg PPT NAcc

  6. SpecificGene inactivation in dopamine neurons DAT Gene DAT BAC 210kb 5’ Homology Cre 3’ Homology iCre TH iCre TH iCre DAT Turiault et al, 2007

  7. Cre Specific expression of ChR2-GFP within DAT expressing neurons Virus injection with a glass pipette in DAT-Cremice Prefrontal cortex VTA Nucleus accumbens Virus AAV1 ChR2-EYFP floxed AAV 1 specificity. AAV wider than lentivirus?

  8. Presentation de la self admin : A partir du dessin final de CL pour ERC grant Inclure le film de SA.

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  10. b. a. c.

  11. Tutu DA stimulation One lever press induced a laser stimulation of 1 minute with that Frequency : 1 sec. 5 pulses, 20 Hz (204ms) For 1’ Silence (130 ms) 5 pulses /5sec  /3sec  /sec  10 pulses/sec  15 pulses/sec What do we know about dopamine self stimulation ? 1. Optical DA stimulation increase food self administration. 2. After extinction, laser had a cue-like reinstatement effect. Model addictive behavior ? De Lecea DA stimulation  Duration : 5s, 20 pulses of 5ms at 25Hz close to a cue for natural reward N.B : One morphine inj (0.2mg/kg)  225nM DA NAcc 20 pulses of 5ms at 25Hz  255nM DA NAcc

  12. With Mister Brown Behavioral sensitization by dopamine stimulation. DA and kyn blockade. Non contingent DA stimulation  Sensitization ? Plasticity ? (easier than yoked) Induce Sensitization by stimulating Glut afference to the VTA and reveal by Coc challenge.

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