actinomyces caries

1. Mouse Anti A. gerencseriae, bacteria from caries Actinomyces naeslundii and oris Hybridoma [532BH2] Anti A. gerencseriae, bacteria from caries Actinomyces naeslundii and oris Hybridoma Lot. No. (See product label) CELL LINE INFORMATION CSC-H1862 Actinomyces naeslundii and oris 532BH2 The antibody is against A. gerencseriae, bacteria from caries Actinomyces naeslundii and oris（Name: Actinomyces gerencseriae strains OMZ 199 (C65 = CCUG 37400), OMZ 672 (WVU 307 = CCUG 35927), 3 own isolates from human root caries and unknown bacteria from tooth caries; Origin: George Bowden, University of Manitoba, Winnipeg, CA; Chemical Composition: intact formalinized bacteria） Very limited molecular epidemiological data are available on the role of Actinomyces spp. in the pathogenesis of caries in the primary dentition. Therefore, we investigated their distribution in supragingival plaque of ethnic Chinese preschool children from Singapore and Hong Kong, either with or without active caries. Plaque samples were taken from intact interproximal enamel areas using dental floss. Bacterial genomic DNA of each sample was extracted and variable regions of 16S ribosomal DNA amplified and labelled with digoxigenin. Oligonucleotide probes specific for Actinomyces bovis, Actinomyces gerencseriae, Actinomyces israelii, Actinomyces meyeri, Actinomyces odontolyticus, catalase-negative Actinomyces naeslundii (genospecies 1 and 2) and catalase-positive Actinomyces naeslundii genospecies 2 (previously Actinomyces viscosus serotype II) were used to detect these species using Southern hybridization with a Minislot and Miniblotter system. A. odontolyticus, A. gerencseriae and A. meyeri were detected with similar frequency in both Singapore and Hong Kong samples or in those with and without active caries. However, the prevalence of A. naeslundii was significantly different in the two locales (p < 0.05). A. odontolyticus (88.7%), A. gerencseriae (56.6%) and A. naeslundii (50.9%) were detected in a majority of the samples and the positive hybridization signals of A. gerencseriae in the caries-active group were stronger than from the caries-free group. A. bovis and A. israelii were undetectable in any of the samples. These data imply that A. odontolyticus, A. naeslundii and A. gerencseriae may play an important role in supragingival plaque formation on primary teeth in ethnic Chinese, with others such as A. meyeri contributing. A. gerencseriae, bacteria from caries Actinomyces naeslundii and oris female balb/c Mouse spleen Mouse SP2/0-Ag14 Mouse X Mouse Hybridoma Mycoplasma Status: Negative (MycoAlert Kit) Cat.No. Common Name Clone Cell Line Description Introduction Immunogen Immunological Donor Myeloma Fusion Species Mycoplasma ANTIBODY INFORMATION Actinomyces naeslundii (all strains tested), and Actinomyces oris (most strains tested) IgG2b Actinomyces naeslundii and oris Cell binding; ELISA; Immunofluorescence Reactivity Isotype Target Application SAFETY AND PACKAGING Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com

2. liquid nitrogen Storage Safety Considerations The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the hybridoma. 3. Wash hands after handling the hybridoma and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. Dry Ice Ship REFERENCES 1.Gmür, R., Wyss, C., Xue, Y., Thurnheer, T., and Guggenheim, B. (2004). Gingival crevice mi crobiota from Chinese patients with gingivitis or necrotizing ulcerative gi ngivitis. Eur. J. Oral Sci. 112, 33-41. Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com