40 likes | 185 Views
Intracellular Signaling Pathways Activated by Endothelins. SIGMA-ALDRICH. Intracellular Signaling Pathways Activated by Endothelins
E N D
Intracellular Signaling Pathways Activated by Endothelins SIGMA-ALDRICH
Intracellular Signaling Pathways Activated by Endothelins Occupation of the endothelin A receptor (ETAR) induces activation of phospholipase C (PLC) which is central to the generation of cellular responses to endothelin 1 (ET-1). PLC cleaves phosphatidylinositol 4,5-bisphosphate (PIP2) to form diacylglycerol (DAG) and D-myo-inositol-1,4,5-trisphosphate (IP3). IP3 regulates intracellular Ca2+ by binding to the IP3 receptor on the endoplasmic reticulum (ER) and stimulating Ca2+ release from the ER stores. DAG binds to and activates protein kinase C (PKC). Increases in the concentration of intracellular Ca2+ is critical for eliciting responses to ETs. The ETA receptor is linked to a Ca2+ channel (CaCh) in the plasma membrane that opens in response to receptor occupation by ET-1 or its agonists, thus further increasing the intracellular Ca2+content. ETs have also been shown to open nonselective cation channels (NsCh) or chloride channels (ClCh) to induce cellular depolarization by increasing in influx of Na+ and efflux of Cl–, respectively. ETs also open potassium channels (KCh) which leads to the passive efflux of K+ and hyperpolarization that, in turn, inhibits the CaCh and Ca2+ influx. References Neylon, C.B, Vascular biology of endothelin signal transduction. Clin. Exp. Pharmacol. Physiol., 26, 149-153 (1999). Masaki, T., et al., Subcellular mechanisms of endothelin action in vascular system. Eur. J. Pharmacol., 375, 133-138 (1999).