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Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Effect of atmospheric deposition of nitrogen on the fixation and assimilation of nitrogen by wild lupine ( Lupinus perennis ) in the sand dunes of Lake Michigan. Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi . Introduction.

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Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

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  1. Effect of atmospheric deposition of nitrogen on the fixation and assimilation of nitrogen by wild lupine (Lupinusperennis) in the sand dunes of Lake Michigan Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

  2. Introduction • Located at the Lake Michigan Dunes • KarnerBlue Butterfly is an endangered species • Sole food source of Karner Blue Butterfly • Increase in Nitrogen Deposition recently • I hypothesize that the addition of nitrogen in the soil will increase the biomass production of wild lupine.

  3. Methods • Germinated in dishes and transplanted • Randomly assigned • NH4NO3 Treatment groups: Water, 7.5g m-2 , 15g m-2 • Harvested 5 months later • Total dry weight biomass and biomass allocation analysis through: • Kruskal-Wallis test • Linear regression analysis

  4. Median total biomass of wild lupine seedlings. Results were significant at p<0.05. n=12. * represents outliers.

  5. Median belowground biomass allocation of wild lupine seedlings. Results were significant at p<0.06. n=12. * represents outliers.

  6. Linear Regression Line showing a correlation between the amount of biomass allocated belowground (vertical axis) and the amount of total biomass (horizontal axis).  

  7. Summary • Contradictory • Population Decline • Total biomass decrease as nitrogen increases • Physiological Drought • Reduction of below ground biomass allocation percent as nitrogen increase • Below Ground Biomass Allocation Percent Increases as Total Biomass Increases • Allomeric growth vs. Nitrogen increase

  8. Acknowledgements This project was funded by Flora Richardson Foundation

  9. Works Cited Driscoll, C., D. withal, J. Amber, E. Boyer, M. Castro, C. Cronan, C. Goodale, P. Groffman, C. Hopkinson, K. Lambert, G. Lawrence, and S. Ollinger. 2003. Nitrogen pollution in the northeastern United States: sources, effects and management options. Bioscience 53: 357-374. Forrester, J. A., D. J. Leopold, and S. D. Hafner. 2005. Maintaining Critical Habitat in a Heavily Managed Landscape: Effects of Power Line Corridor Management on Karner Blue Butterfly (Lycaeidesmelissasamuelis) Habitat. Restoration Ecology 13(3): 488-498. Grundel, R., N. B. Pavlovic, and C. L. Sulzman. 1998. The effect of canopy cover and seasonal change on host plant quality for the endangered Karner blue butterfly (Lycaeidesmelissasamuelis). Oecologia 114: 243-250. Grundel, R., N. B. Pavlovic, and C.L. Sulzman. 1999. Nectar Plant Selection by the Karner Blue Butterfly (Lycaeidesmelissasamuelis) at the Indiana Dunes National Lakeshore. The American Midland Naturalist 114: 1-10.

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