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Exploring Microscopes in Biology: Light vs. Electron

Learn about the different types of microscopes used in biology, including compound light microscopes and electron microscopes, their parts, functions, and how they are used to magnify and analyze specimens.

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Exploring Microscopes in Biology: Light vs. Electron

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  1. In Pg. 14 • What is a light Microscope? Use Pg. 171 • Answer in Blue

  2. Title Pg. 15 Cells – 100pts. If you use color you will receive 25 pts. extra credit • Draw table 7.1 on page 187 • Draw a plant cell and an animal cell along with labeling all of the organelles. • List three differences between plant and animal cells below the drawings you did. • List the three parts of the cell theory and who came up with these. • Draw a phospholipid bilayer and label all parts. • Draw a phospholipid and label parts. • Draw a prokaryotic cell and label all parts

  3. Do it now pg. 14 • Greenhouse gases help keep Earth at a habitable temperature by • A. blocking the cold winds and ices originating in outer space. • B. serving as essential nutrients for atmospheric phytoplankton. • C. allowing only infrared light to reach Earth’s surface. • D. retaining some of the Sun’s energy in our lower atmosphere.

  4. Out Pg. 14 • What are the differences between a plant and an animal cell? • Answer in Blue

  5. IN Pg. 16 • What are the three parts of the cell theory, see page 172 in your text book. • Answer in Blue:

  6. Do it now Pg. 16N.12.A.5 http://www.rpdp.net/sciencetips_v2/N12A5.htm The model below is designed to show how a lunar eclipse occurs. What is the greatest limitation of this model? • The light bulb is standing straight up instead of tilted on an axis. • The comparative sizes and distances are inaccurate. • The shadow is being cast in the wrong direction. • The heat released is much less than that released by the Sun.

  7. Do it now Pg. 16N.12.A.5 http://www.rpdp.net/sciencetips_v2/N12A5.htm The model below is designed to show how a lunar eclipse occurs. What is the greatest limitation of this model? • The light bulb is standing straight up instead of tilted on an axis. • The comparative sizes and distances are inaccurate. • The shadow is being cast in the wrong direction. • The heat released is much less than that released by the Sun.

  8. Tools of Biology Pg. 17 What a biologist works with

  9. Three Types of Microscopes • Compound Light Microscope • Electron Microscopes • Transmission electron Microscope • Scanning electron microsope

  10. Compound Light Microscope T.E.M. S.E.M. Uses two lenses to magnify an image Shines electrons into an image and magnifies it. Shines electrons on the surface of an organism and magnifies it. Microscope Types

  11. Light Microscope • Compound Microscope – meaning both a Eye piece and an Objective. • Compounds means two. • Light Microscopes might have both a mirror or a light source powered by battery or electricity. • Light mircroscopes – some of the first to be used.

  12. Parts of the Microscope • Ocular or eyepiece Coarse adjustment • Objective Stage Clips • Stage Arm • Glass slide • Cover Slip • Diaphram • Base • Fine adjustment

  13. Light Microscope Facts

  14. Resolution – the ability to see between two lines. Magnification – the ability to enlarge an image Resolution Vs. Magnification

  15. Ocular – 10x magnification – part of microscope you look through. Objective lens – normally 3 maybe 4 if microscope has oil emersion. Principal part used for magnification. 4x, 10x, 40x all common light. Oil emersion – used for prokaryotes – 100x Ocular (eyepiece) vs. Objective

  16. Figuring total Magnification • Ocular (eye piece) multiplied by the Objective. • Ex. • 10 X 4 = 40 • 10 X 10 = 100 • 10 X 40 = 400 • 10 X 100 = 1000 (must use oil to focus)

  17. Uses of Light Microscope • To view slides • To help identitify organisms. • To help identify bacteria • To enlarge smaller particles

  18. Electron Microscopes S.E.M.S & T.E.M.S

  19. The Scanning Electron Microscope, or SEM, is an incredible tool for seeing the unseen worlds of microspace.

  20. The Scanning Electron Microscope creates the magnified images by using electrons instead of light waves

  21. Scanning Electron Microscopes • S.E.M. – look only at the outside of an object. • They give a 3-Dimage of the object.

  22. Philips CM 10 Transmission Electron MicroscopeHigh Voltage: 40-100 KVResolution: 0.2nm

  23. Transmission Electron Microscope • Used to look into the cell or into the object. • Produces a 2 – D view of the object.

  24. More Tools For The Laboratory Scientists

  25. Laboratory Techniques • Centrifugation • Micromanipulation • Cell Cultures

  26. Centrifugation • Spinning in a liquid to brake down and separate based on the molecular weight of an object.

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