1 / 13

Mass Spectrometry Facility

Mass Spectrometry Facility. Advanced Analysis Centre University of Guelph. Presented by: Dr. Dyanne Brewer Mass Spectrometry Facility Manager May 2014. MS Sample Submission Workflow. Sample Submission. Submission Forms: http://www.uoguelph.ca/~bmsf/. Training Available:

darin
Download Presentation

Mass Spectrometry Facility

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mass Spectrometry Facility Advanced Analysis Centre University of Guelph Presented by: Dr. Dyanne Brewer Mass Spectrometry Facility Manager May 2014

  2. MS Sample Submission Workflow Sample Submission Submission Forms: http://www.uoguelph.ca/~bmsf/ Training Available: GC-MS derivitization Protein digestion Sample Preparation Training Available: GC-MS LC-MSn Data Acquisition Data Interpretation Training Available: Metabolomics software Proteomics software

  3. Instrumentation Agilent GC-MS Bruker GC-MS-MS Solid, liquid, gas, SPME Bruker LC-MSn Agilent LC-HR MS-MS GC- gas chromatography LC- liquid chromatography MS- mass spectrometry

  4. Sample Type: Small Molecule

  5. Small Molecule GC-MS Sample Collection Metabolite Extraction Compound Derivitization Gas Chromatogram NIST match to Glycine N,N-bis(trimethylsilyl) trimethyl ester Dr. MutchUoG HHNS

  6. Small Molecule Mass Accuracy Dr. MandervilleUoGChem

  7. Small Molecule LC-MS -peonidin -hexose -deoxihexose Dr. Kirakosyan Un. Michigan

  8. Small Molecule LC-MS Heart Bladder kidney Brain Tissue Extract Calibration Curve of Peonidin3-O-glucoside Dr. Kirakosyan Un. Michigan

  9. Metabolomics Workflow Separate and Detect MS GC-MS/LC-MS Compare and Stats MS database search targets Compare to database/standard Target MS/MS In-house Metlin/NIST library or online MassHunter/MPP software ID GC-MS data Pathway?

  10. Sample Type: Large Biomolecule

  11. Protein MW Protein solution Clean-up LC Deconvoluted data Raw data 27,310.4656 amu Dr. Brown UoG Physics

  12. Protein Identification Protein digest LC-Chromatogram Peptide Fragmentation Protein Identification Dr. Merrill UoG MCB

  13. Mass Spectrometry Facility • Advanced Analysis Centre • University of Guelph Manager: Dr. Dyanne Brewer Research Associate: Dr. ArmenCharchoglyan Location: Science Complex Rm 1205 Email: dbrewer@uoguelph.ca Phone: x58649 lab x53793 DB office

More Related