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A. B. C. 1 2 3 4 5. D. 400 bp. 300 bp. gag. LacZ. 200 bp. pNIP46 pBlaF*-gag. pBU pBlaF*-lacZ. Ms6 derived vectors. hyg B. aph. attP. attP mut. E. X. X. BCG 1173P2. attB tRNA alaV. attB like tRNA alaU. BCG::pNIP46::pBULacZ. hyg B.
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A B C 1 2 3 4 5 D 400 bp 300 bp gag LacZ 200 bp pNIP46 pBlaF*-gag pBU pBlaF*-lacZ Ms6 derived vectors hygB aph attP attPmut E X X BCG 1173P2 attB tRNAalaV attBlike tRNAalaU BCG::pNIP46::pBULacZ hygB aph gag LacZ B/P B/P Blike/Pmut Blike/Pmut