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Aminoacyl tRNA synthetase (aaRS)

Aminoacyl tRNA synthetase (aaRS). Oleh: Ertanti Rizki P. 20512033 Suci Rizki Nurul Aeni 20512044. KK Biokimia-Institut Teknologi Bandung. Outline. T erdapat dalam sel makhluk hidup, yaitu pada Ribosom. What is aa RS ?. Berperan dalam proses translasi RNA.

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Aminoacyl tRNA synthetase (aaRS)

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  1. Aminoacyl tRNA synthetase (aaRS) Oleh: Ertanti Rizki P. 20512033 Suci Rizki Nurul Aeni 20512044 KK Biokimia-Institut Teknologi Bandung

  2. Outline

  3. Terdapat dalam sel makhluk hidup, yaitu pada Ribosom What is aa RS ? Berperan dalam proses translasi RNA Pengikatanasam amino spesifikketRNA

  4. Tahapan pengikatantRNAdenganasamamino • Sisiaktifenzimmengikatasam amino dan molekulATP. 2) ATP kehilanganduagugusfosfatdan bergabungdenganasam amino sebagaiAMP. 3) tRNAberikatankovalendenganasam amino menggeserAMP disisiaktifenzim. 4) EnzimmelepaskantRNA-aminoasil, disebut juga“asam amino teraktivasi”

  5. Mekanisme

  6. Perbedaan Kelas Asam Amino

  7. Kinetika

  8. kinetic of Enzyme • Suhu • Konsentrasi enzim • Konsentrasi Substrat • Inhibitor • Jumlah Pyrophosfat

  9. What the development? • Inhibitor enzim ini dapat berguna bagi treatment untuk beberapa penyakit antara lain Malaria dan HIV 1 • Inhibitor enzim ini berpotensi untuk zat antibiotik

  10. Spesific Enzyme Name of IUBMB • EC 6 : Class Ligase • EC 6.1 : Subclass Forming Carbon-Oxygen Bonds • EC 6.1.1 : Ligases forming aminoacyl-tRNA and related compounds • EC 6.1.1.11 : Serine-tRNAligase • Nama lain : seryl-tRNA synthetase; SerRS; seryl-transfer ribonucleate synthetase; • seryl-Transfer RNA synthetase; seryl-transfer ribonucleic acid synthetase; serine translase • NamaSistematik: L-serine:tRNASer ligase (AMP-forming)

  11. Serine-tRNAligase Fungtion • Mengakatalisispengikatankovalendariserinkegugus 3’-OH darinukleotidatRNA-nyadalamsuatuproses yang digerakanolehhidrolisis ATP.

  12. Reaction of Serine-tRNAligase ATP + L-serine + tRNASer AMP + diphosphate + L-seryl-tRNASer

  13. Characteristic of Serine-tRNAligase • Terdapatduadomain yang terikat pada active side dan pada antikodon • Domain N-terminal terdiridarirantai yang sangatpanjang • Seryl-tRNAsynthetasememilikisetidaknya lima spesies yang berbeda yang secarakolektifmengakuienamkodonberbedauntukasam amino

  14. Kinetic of Serine-tRNAligase

  15. Application of Serine-tRNAligase

  16. Modification of Serine-tRNAligase

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