1 / 29

How to get the cloned gene? (How to clone or identify the gene?)

How to get the cloned gene? (How to clone or identify the gene?). (1) From academic scientist. (2) From company. (3) PCR (RT-PCR). (4) From DNA library (Hybridization). (5) From expression library (Antibody). (6) From expression library (Protein Activity). (7) Microarray analysis.

davischarles
Download Presentation

How to get the cloned gene? (How to clone or identify the gene?)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. How to get the cloned gene? (How to clone or identify the gene?) (1)From academic scientist (2)From company (3)PCR (RT-PCR) (4)From DNA library (Hybridization) (5)From expression library (Antibody) (6)From expression library (Protein Activity) (7) Microarray analysis (8) Mutant (Complement or Rescue)

  2. Figure 4.12. Southern hybridization. (A) Transfer of DNA from the gel to the membrane. (B) The membrane is probed with a radioactively labeled DNA molecule. On the resulting autoradiograph, one hybridizing band is seen in lane 2, and two in lane 3.

  3. Northern Blot Hybridization

  4. Primer Extension Analysis

  5. S1 Nuclease Mapping Analysis

  6. Reporter Gene Assay

  7. PITX2-LACZ Staining of mouse embryo day 11 PITX2: bicoid-related transcription factor

  8. Luciferase gene Luciferase Activity (Promega)

More Related