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18S rRNA clone library of phytoplankton in the Columbia River and its coastal zone

18S rRNA clone library of phytoplankton in the Columbia River and its coastal zone. By Pete Kahn Mentors: Lydie Herfort and Peter Zuber. http://www.mapwatch.com/news-blog/images/phytoplankton.jpg. Significance of phytoplankton. Major producers: O 2 : 90% CO 2 =>C 3 : 50%

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18S rRNA clone library of phytoplankton in the Columbia River and its coastal zone

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  1. 18S rRNA clone library of phytoplankton in the Columbia River and its coastal zone By Pete Kahn Mentors: Lydie Herfort and Peter Zuber http://www.mapwatch.com/news-blog/images/phytoplankton.jpg

  2. Significance of phytoplankton • Major producers: O2: 90% CO2=>C3: 50% • Base of aquatic food chain

  3. Interactions with other Domains BacterioplanktonColonization : POC=>DOC Competition for NO32- and NH3? http://www.fossilmuseum.net/Tree_of_Life/Domains_Archaea_Bacteria/Domains_of_life.jpg

  4. Significance: Why is CMOP interested in Phytoplankton? • Base of aquatic food chain • Major producers: O2: 90%; CO2=>C3: 50% • Interaction with other two Domains -Crenarchaeota: “competition” -Bacterioplankton: colonization -POC=>DOC Marine Phytoplankton bloom off the coast of Argentina http://www.mapwatch.com/news-blog/images/phytoplankton.jpg

  5. Traditional Methods Flow cytometry Microscopy: Pediastrium • Microscopy • Flow Cytometry • Chlorophyll a • Photosynthesis: O2 production C14 uptake http://upload.wikimedia.org/wikipedia/commons/c/c7/Picoplancton_cytometrie.jpg http://www.keweenawalgae.mtu.edu/ALGAL_IMAGES/chlorophyceans/Pediastrium_n16_dollartow3_402_c.jpg Filtering for chlorophyll a

  6. Objectives • 18S rRNA clone library for different seasons and different locations: 0 PSU, 30 PSU for spring and summer; compare to winter samples • Compare with Bacteria & Archaea clone libraries from Dan Murphy • Identify unexpected changes • Monitor health of ecosystem Pediastrium http://www.keweenawalgae.mtu.edu/ALGAL_IMAGES/chlorophyceans/Pediastrium_n16_dollartow3_402_c.jpg

  7. Methods Overview Understand Phytoplankton community in estuary Phylogenetic analysis Sequence analysis w/ BLAST Yan, Q. Y., Yu, Y. H. & Feng, W. S. (2006). Genetic fingerprinting of plankton community provides new insights into aquatic ecology. Progress in Natural Science16, 893-898.

  8. Methods Overview Water sample => Sterivex on site Total nucleic acid extraction from sterivex in lab Amplification through PCR

  9. Variations in nucleic acid in environmental samples Oct 2006 Feb 2007 • Between seasons • Between locations April Wecoma & Forerunner: Freshwater << Surface Coastal DNA RNA

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