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Mastering Gene Isolation Techniques for Transgenic Organisms

Explore gene isolation and manipulation methods like microinjection, PCR, sequencing, and transgene introduction for plant and animal research. Learn about the Ti plasmid vector, T-DNA transmission, and more.

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Mastering Gene Isolation Techniques for Transgenic Organisms

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  1. Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.

  2. Finding specific nucleic acids by using gel electrophoresis and blotting

  3. Polymerase chain reaction (PCR)

  4. Sequencing

  5. Sanger Sequencing (Dideoxy Sequencing) The structure of 2’,3’-dideoxynucleotides

  6. The dideoxy sequencing method

  7. Reading the DNA sequence from an automatic sequencer

  8. How to amplify an interesting gene

  9. Making a cDNA clone

  10. Introduction of a transgene

  11. Agrobacterium tumefaciens causal agent of Crown Gall

  12. The Ti plasmid vector

  13. The generation of a transgenic plant

  14. The generation of a transgenic plant

  15. Pattern of transmission of T-DNA

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